Udermann H, Schuhmann G
Z Rechtsmed. 1975 Nov 11;76(2):119-22. doi: 10.1007/BF00200556.
An improved method for detection of diatoms in case of drowning is reported. The sample is digested with nitric acid. In order to avoid destruction of diatoms as well as losses by centrifugation etc. the time for wet digestion was reduced and the digest solution is filtered through a membrane filter. Fatty material is removed by alternate washing the filter with 2-Propanol and Petroleum ether. Following the wet digestion of the filter, aliqutos of this digest solution are filtered and the filter is examined microscopically. As the recommended procedure is less time consuming and yields almost complete recovery of diatoms the method has great probative value in case of death by drowning.
本文报道了一种改进的溺水案例中硅藻检测方法。样本用硝酸消化。为避免硅藻被破坏以及因离心等造成损失，缩短了湿式消化时间，消化液通过膜滤器过滤。用异丙醇和石油醚交替洗涤滤器以去除脂肪物质。滤器湿式消化后，取该消化液的 aliquots 进行过滤，并对滤器进行显微镜检查。由于推荐的方法耗时较少且硅藻回收率几乎达到 100%，该方法在溺水死亡案例中具有很大的证明价值。
