McDoniels-Silvers Amy L, Stoner Gary D, Lubet Ronald A, You Ming
Department of Pathology, Medical College of Ohio, Toledo, OH 43614, USA.
Neoplasia. 2002 Mar-Apr;4(2):141-50. doi: 10.1038/sj.neo.7900217.
The Atlas human cDNA expression array was used to evaluate gene expression profile changes in the genesis of human lung adenocarcinomas and squamous cell carcinomas. Gene expression changes between adenocarcinomas and squamous cell carcinomas were also analyzed. Of the 588 gene targets, 262 genes were expressed in these tissues and, of these, 45 genes were differentially expressed by at least two-fold in tumor tissues compared to corresponding normal tissues. Semiquantitative reverse-transcriptase polymerase chain reaction was used to confirm gene expression changes. Only those genes that reflected changes in >50% of the analyzed tissues were included in the final analysis. Ultimately, 26 genes were evaluated with 14 genes overexpressed and 12 genes underexpressed compared to matching normal lung tissues. Although similar expression changes were detected in adenocarcinomas and squamous cell carcinomas for most of the genes analyzed, some subtype-specific differences were also found. Genes encoding cell cycle regulators, intracellular signal transducers, cell receptor and adhesion molecules, growth factors, oncogenes, and apoptotic effectors were differentially expressed in this study. These gene expression changes may directly contribute to the initiation or progression of human lung cancer or may be secondary effects of the tumorigenesis process. Regardless, many of these differences may be useful in the diagnosis and/or treatment of this deadly disease.
采用Atlas人类cDNA表达芯片评估人肺腺癌和鳞状细胞癌发生过程中的基因表达谱变化。同时分析腺癌和鳞状细胞癌之间的基因表达差异。在588个基因靶点中，有262个基因在这些组织中表达，其中45个基因在肿瘤组织中的表达与相应正常组织相比至少相差两倍。采用半定量逆转录聚合酶链反应来确认基因表达变化。最终分析仅纳入那些在所分析组织中50%以上呈现变化的基因。最终，对26个基因进行了评估，与匹配的正常肺组织相比，14个基因过表达，12个基因低表达。尽管在所分析的大多数基因中，腺癌和鳞状细胞癌检测到相似的表达变化，但也发现了一些亚型特异性差异。本研究中编码细胞周期调节因子、细胞内信号转导分子、细胞受体和黏附分子、生长因子、癌基因和凋亡效应分子的基因存在差异表达。这些基因表达变化可能直接促进人肺癌的发生或进展，也可能是肿瘤发生过程的继发效应。无论如何，其中许多差异可能对这种致命疾病的诊断和/或治疗有用。
