Rubin B, Alibaud L, Huchenq-Champagne A, Arnaud J, Toribio M-L, Constans J
Unité de Physiopathologie Cellulaire et Moléculaire, CNRS UPR-2163, Hôpital Purpan, 31059 Toulouse Cedex 03, France.
Scand J Immunol. 2002 Feb;55(2):111-8. doi: 10.1046/j.1365-3083.2002.01044.x.
Several models are proposed for T-cell antigen receptor (TCR) assembly and structure. However, there is little experimental data favouring directly either one or the other(s). The minimal complex appears to be composed of a TCRalphabeta/CD3deltaepsilon,gammaepsilon/zeta2 structure but at the cell membrane, multimers of this minimal structure may be formed. Quantitative cytofluometry has suggested three CD3epsilon chains for two TCRbeta (or TCRdelta) chains/complex. Such data should be repeated with monoclonal antibodies (MoAb) against extracellular (EC) parts of CD3delta or CD3gamma chains. In the present review, we have compared the TCR/CD3 assembly of pre-TCR, TCRgammadelta and TCRalphabeta containing complexes, and analysed the reactivity of antibodies (Abs) against the EC part of CD3delta chains. Our data suggest an alternative assembly pathway and structure of TCR/CD3 complexes.
针对T细胞抗原受体（TCR）的组装和结构提出了几种模型。然而，几乎没有实验数据能直接支持其中某一种或其他几种模型。最小复合物似乎由TCRαβ/CD3δε、γε/ζ2结构组成，但在细胞膜上，这种最小结构可能会形成多聚体。定量细胞荧光测定法表明，两个TCRβ（或TCRδ）链/复合物对应三条CD3ε链。此类数据应用针对CD3δ或CD3γ链细胞外（EC）部分的单克隆抗体（MoAb）进行重复验证。在本综述中，我们比较了含前TCR、TCRγδ和TCRαβ复合物的TCR/CD3组装情况，并分析了针对CD3δ链EC部分的抗体（Abs）的反应性。我们的数据提示了TCR/CD3复合物的另一种组装途径和结构。
