Esteves Aida, Parreira Ricardo, Venenno Teresa, Franco Margarida, Piedade João, Germano De Sousa José, Canas-Ferreira Wanda F
Unidade de Virologia/Unidade de Parasitologia e Microbiologia Médicas, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, P-1349-008 Lisbon, Portugal.
AIDS Res Hum Retroviruses. 2002 Mar 20;18(5):313-25. doi: 10.1089/088922202753519089.
In this study, we have investigated the diversity of current HIV-1 strains circulating in the metropolitan area of Lisbon, Portugal. A total of 217 HIV-1-positive blood samples, collected between October 1998 and December 2000, was genetically characterized in the gp120 C2V3C3 region (n = 205) or part of the gp41 N-terminal segment (n = 12) by heteroduplex mobility assay (HMA) and/or DNA sequencing. The HMA subtyping efficiency (number of samples unambiguously subtyped by HMA divided by the total number of samples subtyped) was 65.9% (143 of 217), with indeterminate migration patterns of subtype A and G strains contributing significantly to this value. On the overall, subtype B was the most prevalent (50.2%), followed by subtypes G (21.7%), A (17.5%), and F (5.5%), whereas subtypes C, D, H, and J accounted altogether for 5.1% of the infections. Non-B subtypes were responsible for 77.4 and 33.1% of the infections among African immigrants and Portuguese subjects, respectively. Angolan individuals (n = 25) were the only ones infected with all the HIV-1 subtypes documented, probably reflecting a high degree of viral genetic diversification in their country of origin. Phylogenetic analysis showed a predominance of IbNG-like viruses among subtype A sequences and two new major subclusters within subtype G (G(P) and G(P)'). The majority of the Portuguese G sequences described formed a well-defined subcluster (G(P)), supported by bootstrap values >90%, phylogenetically distant from clade G sequences in databases. gag (p24/p7) sequence analysis of these variants confirmed the maintenance of the subtype G subclusters. The multiple subclustering observed for the major clades A, B, D, and G, as well as the variety of subtypes found, indicate a high diversity of HIV-1 variants circulating in Portugal and suggest a need for continuous epidemiologic surveillance.
在本研究中，我们调查了葡萄牙里斯本大都市区当前流行的HIV-1毒株的多样性。1998年10月至2000年12月期间收集的总共217份HIV-1阳性血液样本，通过异源双链迁移分析（HMA）和/或DNA测序，在gp120 C2V3C3区域（n = 205）或gp41 N端片段的一部分（n = 12）进行了基因特征分析。HMA亚型分型效率（通过HMA明确分型的样本数除以分型的样本总数）为65.9%（217份中的143份），A型和G型毒株不确定的迁移模式对该值有显著影响。总体而言，B型是最常见的（50.2%），其次是G型（21.7%）、A型（17.5%）和F型（5.5%），而C型、D型、H型和J型总共占感染的5.1%。非B型亚型分别占非洲移民和葡萄牙受试者感染的77.4%和33.1%。安哥拉个体（n = 25）是唯一感染了所有记录的HIV-1亚型的人群，这可能反映了其原籍国高度的病毒基因多样化。系统发育分析显示，A型序列中IbNG样病毒占优势，G型中有两个新的主要亚群（G(P)和G(P)'）。所描述的大多数葡萄牙G型序列形成了一个明确的亚群（G(P)），自展值>90%支持该亚群，在系统发育上与数据库中的G进化枝序列相距甚远。对这些变体的gag（p24/p7）序列分析证实了G型亚型的维持。在主要进化枝A、B、D和G中观察到的多个亚群聚类，以及发现的多种亚型，表明葡萄牙流行的HIV-1变体具有高度多样性，并提示需要持续进行流行病学监测。
