Lasky M, Perret J L, Peeters M, Bibollet-Ruche F, Liegeois F, Patrel D, Molinier S, Gras C, Delaporte E
Retrovirus Laboratory, ORSTOM, Montpellier, France.
AIDS. 1997 Jan;11(1):43-51. doi: 10.1097/00002030-199701000-00007.
To identify the genetic subtypes and characteristics of HIV-1 strains from individuals infected after overseas deployment.
Sixty-one HIV-1-positive individuals detected between 1986 and 1995 in the French army were included in the study. For each patient, the year and country of HIV infection are known. Genetic subtypes of HIV-1 were determined using the heteroduplex mobility assay (HMA) using ED5/ED12 as outer and ES7/ES8 as inner primers. Strains were further characterized by sequencing and phylogenetic analysis of the C2-V3 region. The amino-acid sequences corresponding to the V3 region were aligned on the basis of the subtyping results and were then compared to the consensus V3 sequences of the corresponding subtypes.
Among the 61 patients studied, nine became infected in France, and 52 were HIV-negative before overseas deployment but HIV-positive at their return. The majority (n = 43) deployed in Africa and a limited number of patients deployed in Asia (Cambodia, n = 5) or South America (guyana, n = 4). The nine individuals who were not deployed overseas were all infected with subtype B strains. The majority of the other patients were infected with non-B strains; eight subtype A, 20 subtype B, 16 subtype C, one subtype D, six subtype E and one subtype F. Five of the six subtype E strains were contracted in Cambodia and one in Djibouti, and all subtype C strains were from Djibouti. Phylogenetic analysis revealed a large diversity among the different strains introduced into France. Analysis of the amino-acid sequences of the V3 loop revealed the introduction of uncommon V3-loop patterns.
In the group of HIV-1-infected individuals that we studied and who were deployed overseas, 63.4% were infected with non-B strains. In addition, the subtype A, B and C viruses in this population were very heterogeneous. Due to the routine occurrence of international travel and deployment, the predominance of subtype B HIV-1 viruses may change in European countries. However, the possible implications on the dynamics of the HIV-1 epidemic needs further follow-up.
鉴定海外部署后感染个体中HIV-1毒株的基因亚型及特征。
本研究纳入了1986年至1995年间在法国军队中检测出的61例HIV-1阳性个体。对于每位患者，HIV感染的年份和国家均已知。使用以ED5/ED12为外侧引物、ES7/ES8为内侧引物的异源双链迁移率分析（HMA）来确定HIV-1的基因亚型。通过对C2-V3区域进行测序和系统发育分析，进一步对毒株进行特征描述。根据亚型分析结果对与V3区域对应的氨基酸序列进行比对，然后与相应亚型的V3共有序列进行比较。
在研究的61例患者中，9例在法国感染，52例在海外部署前HIV阴性但回国时HIV阳性。大多数（n = 43）部署在非洲，少数患者部署在亚洲（柬埔寨，n = 5）或南美洲（圭亚那，n = 4）。未进行海外部署的9例个体均感染了B亚型毒株。其他大多数患者感染的是非B亚型毒株；8例为A亚型，20例为B亚型，16例为C亚型，1例为D亚型，6例为E亚型，1例为F亚型。6例E亚型毒株中的5例在柬埔寨感染，1例在吉布提感染，所有C亚型毒株均来自吉布提。系统发育分析显示引入法国的不同毒株之间存在很大差异。对V3环氨基酸序列的分析揭示了不常见的V3环模式的引入。
在我们研究的海外部署的HIV-1感染个体群体中，63.4%感染的是非B亚型毒株。此外，该人群中的A、B和C亚型病毒非常异质。由于国际旅行和部署的常规发生，欧洲国家中B亚型HIV-1病毒的优势地位可能会发生变化。然而，对HIV-1流行动态的潜在影响需要进一步随访。
