Yatsuda A P, Kooyman F N J, Ploeger H W, Vieira-Bressan M C R, de Vries E, Eysker M
Department of Infectious Diseases and Immunology, Division of Parasitology and Tropical Veterinary Medicine, Utrecht University, P.O. Box 80165, 3508 TD Utrecht, The Netherlands.
Vet Parasitol. 2002 Apr 30;105(2):131-8. doi: 10.1016/s0304-4017(02)00004-3.
Three groups of four calves each were trickle infected with three different levels of Cooperia punctata: 310 (group A), 1250 (group B) and 5000 (group C) third stage infective larvae (L3) twice a week over a 17-week period. Group D was the non-infected control group. Parasitological parameters as faecal egg counts (epg), worm burdens, size of worms and number of eggs per female were collected and the differences between the groups compared. Serological analyses were also conducted to investigate the efficiency of a recombinant C. oncophora CoES 14.2kDa protein in an ELISA to detect C. punctata antibodies. Group C had higher faecal egg counts until week 9 when the values decreased to those in group B. Mean faecal egg counts in group A were always lower than in the two other infected groups. The worm burdens were highest in group C, and lowest in group A, although the number of worms as a percentage of total larval intake was higher for the lower group. The mean length of the worms was shorter and the number of eggs per female lower for group C than for both other groups. ELISA using the CoES 14.2kDa proved to be efficient in measuring C. punctata antibodies. For group C it took 4 weeks to get increased levels of antibodies and this was one and 2 months more for groups B and A, respectively. Overall, there was a congruent relation between C. punctata antibodies and the cumulative exposure to the three different levels of trickle infections.
310条（A组）、1250条（B组）和5000条（C组）第三期感染性幼虫（L3），每周两次，持续17周。D组为未感染的对照组。收集粪便虫卵计数（epg）、蠕虫负荷、蠕虫大小和每头雌虫的虫卵数等寄生虫学参数，并比较各组之间的差异。还进行了血清学分析，以研究重组牛环形泰勒虫CoES 14.2kDa蛋白在ELISA中检测点状库珀线虫抗体的效率。C组在第9周前粪便虫卵计数较高，之后数值降至B组水平。A组的平均粪便虫卵计数始终低于其他两个感染组。蠕虫负荷在C组最高，在A组最低，尽管较低感染组的蠕虫数量占总幼虫摄入量的百分比更高。C组蠕虫的平均长度比其他两组短，每头雌虫的虫卵数也比其他两组少。使用CoES 14.2kDa进行的ELISA被证明在检测点状库珀线虫抗体方面是有效的。C组需要4周时间抗体水平升高，B组和A组分别多1个月和2个月。总体而言，点状库珀线虫抗体与三种不同水平滴注感染的累积暴露之间存在一致关系。
