Tian Ying, Nie Wen-Hui, Wang Jin-Huan, Yang Yun-Fei, Yang Feng-Tang
Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China.
Yi Chuan Xue Bao. 2002 Feb;29(2):124-7.
We have established a comparative chromosome map between red panda (Ailurus fulgens, 2n = 36) and dog by chromosome painting with biotin-labelled chromosome-specific probes of the dog. Dog probes specific for the 38 automates delineated 71 homologous segments in the metaphase chromosomes of red panda. Of the 38 autosomal paints, 18 probes each delineated one homologous segment in red panda genome, while the other 20 ones each detected two to five homologous segments. The dog X chromosome-specific paint delineated the whole X chromosome of the red panda. The results indicate that at least 28 fissions (breaks), 49 fusions and 4 inversions were needed to "convert" the dog karyotype to that of the red panda, suggesting that extensive chromosome rearrangements differentiate the karyotypes of red panda and dog. Based on the established comparative chromosome homologies of dog and domestic cat, we could infer that there were 26 segments of conserved synteny between red panda and domestic cat. Comparative analysis of the distribution patterns of conserved segments defined by dog paints in red panda and domestic cat genomes revealed at least 2 cryptic inversions in two large chromosomal regions of conserved synteny between red panda and domestic cat. The karyotype of red panda shows high degree of homology with that of domestic cat.
我们利用生物素标记的犬染色体特异性探针进行染色体涂染，建立了小熊猫（小熊猫属，2n = 36）与犬之间的比较染色体图谱。针对犬38条常染色体的特异性探针在小熊猫中期染色体上划定了71个同源区段。在38条常染色体涂染探针中，18条探针各自在小熊猫基因组中划定了一个同源区段，而另外20条探针各自检测到了两到五个同源区段。犬X染色体特异性涂染探针划定了小熊猫的整条X染色体。结果表明，要将犬的核型“转换”为小熊猫的核型，至少需要28次断裂、49次融合和4次倒位，这表明广泛的染色体重排使小熊猫和犬的核型产生了差异。基于已建立的犬与家猫的比较染色体同源性，我们可以推断小熊猫与家猫之间存在26个保守同线区段。对犬涂染探针在小熊猫和家猫基因组中所定义的保守区段分布模式进行比较分析，发现在小熊猫和家猫保守同线的两个大染色体区域中至少存在2次隐匿倒位。小熊猫的核型与家猫的核型显示出高度同源性。
