Mukouyama Etsuko B, Ohsawa Hiromichi, Suzuki Haruo
Department of Biosciences, School of Science, Kitasato University, Kanagawa, Japan.
J Protein Chem. 2002 Jan;21(1):59-64. doi: 10.1023/a:1014135216860.
Sarcosine oxidase from Corynebacterium sp. U-96 is a heterotetrameric enzyme that was reported to contain 1 mol of covalently bound FAD and 1 mol of non-covalently-bound FAD. This work describes the result of reinvestigation of the cofactors in this enzyme. The enzyme was found to contain 1 mol of non-covalently-bound NAD+, 1 mol of non-covalently-bound FAD, and 1 mol of covalent FMN. The covalent FMN was identified by the mass and amino acid sequence analyses of the flavin peptide.
来自棒状杆菌属U-96的肌氨酸氧化酶是一种异源四聚体酶，据报道它含有1摩尔共价结合的黄素腺嘌呤二核苷酸（FAD）和1摩尔非共价结合的FAD。这项工作描述了对该酶中辅因子重新研究的结果。发现该酶含有1摩尔非共价结合的烟酰胺腺嘌呤二核苷酸（NAD+）、1摩尔非共价结合的FAD和1摩尔共价结合的黄素单核苷酸（FMN）。通过对黄素肽的质量和氨基酸序列分析鉴定出了共价FMN。
