Guelev Vladimir, Sorey Steven, Hoffman David W, Iverson Brent L
Department of Chemistry and Biochemistry, The University of Texas at Austin, Austin, TX 78722, USA.
J Am Chem Soc. 2002 Mar 27;124(12):2864-5. doi: 10.1021/ja016834e.
We have been investigating a modular, threading DNA polyintercalator design based upon the 1,4,5,8-naphthalene tetracarboxylic diimide (NDI) intercalating unit. Previously, we have reported the NMR analysis of a bis-intercalator-DNA complex in which the peptide linker between NDI units was found to occupy the DNA major groove (Guelev, Lee, Sorey, Hoffman, Iverson, Chem. Biol. 2001, 8, 415-425). Here we describe the NMR analysis of a complex between a related bis-intercalator known to display altered DNA sequence specificity. In this case, the linker resides in the DNA minor groove. We have thus shown that within this set of sequence specific bis-intercalators, both DNA grooves can be accessed, setting the stage for longer threading polyintercalators designed to have linkers occupying both grooves in an alternating fashion.
我们一直在研究一种基于1,4,5,8-萘四羧酸二亚胺（NDI）嵌入单元的模块化、穿线式DNA多嵌入剂设计。此前，我们报道了一种双嵌入剂-DNA复合物的核磁共振分析，其中发现NDI单元之间的肽接头占据了DNA大沟（Guelev、Lee、Sorey、Hoffman、Iverson，《化学生物学》，2001年，第8卷，第415-425页）。在此，我们描述了一种相关双嵌入剂与已知显示出改变的DNA序列特异性的复合物的核磁共振分析。在这种情况下，接头位于DNA小沟中。因此，我们表明，在这组序列特异性双嵌入剂中，可以进入DNA的两条沟，为设计具有以交替方式占据两条沟的接头的更长穿线多嵌入剂奠定了基础。
