Ross J S, Malamud D, Caulfield J A, Malt R A
Am J Physiol. 1975 Oct;229(4):952-4. doi: 10.1152/ajplegacy.1975.229.4.952.
Using [5-3H]orotic acid and [5-3H]uridine as precursors, we compared the efficiency of labeling and the localization of labeled RNA during compensatory hypertrophy of the mouse kidney. [5-3H]orotic acid in tubules labeled RNA 15 times more intensely that [5-3H]uridine, presumably because of greater incorporation of orotic acid into tubular cells. Of the orotic acid label, 97% was in tubular cells, mostly in the proximal tubules. Only about 80% of the uridine label was in the tubules; the ratio in proximal tubules compared with that in distal tubules was 2:1. No changes in distribution within the nephron were produced during compensatory hypertrophy. [5-3H]uridine should be used as the precursor of generalized labeling is desired, but [5-3H5orotic acid is the better precursor of RNA for many studies of compensatory hypertrophy since it is more efficient and concentrates in the segments of greatest biologic activity.
以[5-³H]乳清酸和[5-³H]尿苷作为前体，我们比较了在小鼠肾脏代偿性肥大过程中标记RNA的效率以及标记RNA的定位。肾小管中的[5-³H]乳清酸标记RNA的强度比[5-³H]尿苷高15倍，这可能是因为乳清酸更多地掺入肾小管细胞。乳清酸标记中，97%存在于肾小管细胞中，主要是近端小管。尿苷标记只有约80%存在于肾小管中；近端小管与远端小管中的比例为2:1。在代偿性肥大过程中，肾单位内的分布没有变化。如果需要进行全身标记，应使用[5-³H]尿苷作为前体，但对于许多代偿性肥大研究来说，[5-³H]乳清酸是更好的RNA前体，因为它更有效且集中在生物活性最高的节段。
