Rastogi N, Venkitasubramanian T A
J Gen Microbiol. 1979 Dec;115(2):517-21. doi: 10.1099/00221287-115-2-517.
Cell wall-deficient forms of Mycobacterium smegmatis were produced in growth medium containing D-cycloserine and horse serum. These cells were transformed into protoplasts with EDTA and lysozyme. Subsequent lysis by nucleases followed by osmotic shock produced membrane vesicles (whole cell ghosts).
耻垢分枝杆菌的细胞壁缺陷型在含有D-环丝氨酸和马血清的生长培养基中产生。这些细胞用乙二胺四乙酸（EDTA）和溶菌酶转化为原生质体。随后用核酸酶裂解，接着进行渗透压休克，产生了膜泡（全细胞空壳）。
