阻断转化生长因子-β受体信号传导可下调瘢痕疙瘩成纤维细胞中转化生长因子-β1的自身分泌。
Blocking transforming growth factor-beta receptor signaling down-regulates transforming growth factor-beta1 autoproduction in keloid fibroblasts.
作者信息
Liu Wei, Cai Zehao, Wang Danru, Wu Xiaoli, Cui Lei, Shang Qingxin, Qian Yunliang, Cao Yilin
机构信息
Department of Plastic and Reconstructive Surgery, Shanghai Institute of Plastic and Reconstructive Surgery, Tissue Engineering Center, Shanghai 9th People's Hospital, Shanghai Second Medical University, Shanghai 200011, China.
出版信息
Chin J Traumatol. 2002 Apr;5(2):77-81.
OBJECTIVE
To study transforming growth factor-beta1 (TGF-beta1) autoproduction in keloid fibroblasts and the regulation effect of blocking TGF-beta intracellular signaling on rhTGF-beta1 autoproduction.
METHODS
Keloid fibroblasts cultured in vitro were treated with either rhTGF-beta1 (5 ng/ml) or recombinant adenovirus containing a truncated type II TGF-beta receptor gene (50 pfu/cell). Their effects of regulating gene expression of TGF-beta1 and its receptor I and II were observed with Northern blot.
RESULTS
rhTGF-beta1 up-regulated the gene expression of TGF-beta1 and receptor I, but not receptor II. Over-expression of the truncated receptor II down-regulated the gene expression of TGF-beta1 and its receptor I, but not receptor II.
CONCLUSIONS
TGF-beta1 autoproduction was observed in keloid fibroblasts. Over-expression of the truncated TGFbeta receptor II decreased TGF-beta1 autoproduction via blocking TGF-beta receptor signaling.
目的
研究瘢痕疙瘩成纤维细胞中转化生长因子-β1（TGF-β1）的自身分泌情况，以及阻断TGF-β细胞内信号传导对重组人TGF-β1（rhTGF-β1）自身分泌的调节作用。
方法
体外培养的瘢痕疙瘩成纤维细胞分别用rhTGF-β1（5 ng/ml）或含截短型II型TGF-β受体基因的重组腺病毒（50 pfu/细胞）处理。采用Northern印迹法观察它们对TGF-β1及其I型和II型受体基因表达的调节作用。
结果
rhTGF-β1上调了TGF-β1和I型受体的基因表达，但未上调II型受体的基因表达。截短型II型受体的过表达下调了TGF-β1及其I型受体的基因表达，但未下调II型受体的基因表达。
结论
在瘢痕疙瘩成纤维细胞中观察到TGF-β1自身分泌现象。截短型TGF-β受体II的过表达通过阻断TGF-β受体信号传导降低了TGF-β1自身分泌。
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