Tatsumi N, Tsuda I, Fukumori T, Hino M, Takubo T, Kondo H
Department of Clinical & Laboratory Medicine, Osaka City University Medical School, Osaka, Japan.
Osaka City Med J. 2001 Dec;47(2):177-88.
In the modern medical laboratory system, simple and rapid processing of specimens are required. In the current system with the transportation line, its centrifugation part would disturb smooth flow of the testing because it needs much time for the centrifugation. To solve the problems, a serum separation method was tried for the whole blood specimen using poly-L-lysine, concanavalin A and phyto-hemoagglutinin. Ploy-L-lysine with molecular weight 130,000 to 210,000 in a final concentration of 0.1% could accelerate blood sedimentation, although its supernatant contaminated platelets. Concanavalin and phytohemoagulutinin could accelerate the sedimentation and obtained plasma, but the method could yield enough amount of supernatant by 1 hour standing. As the purpose of this study is to develop a centrifugeless method, a sieve method using a steel mesh and a magnet was applied to the mixture of EDTA blood, red-cell adhesives and thrombin. The method was unique to separate plasma, but the yield was not so high and chemistry data were not fitted with serum data in some of tests. Thus, the trial would be a new technology, but it was judged that some further improvement will be needed technically.
在现代医学检验系统中，需要对标本进行简单快速的处理。在当前带有运输线的系统中，其离心部分会干扰检测的顺畅流程，因为离心需要很长时间。为了解决这些问题，尝试了一种使用聚-L-赖氨酸、伴刀豆球蛋白A和植物血凝素对全血标本进行血清分离的方法。终浓度为0.1%、分子量为130,000至210,000的聚-L-赖氨酸可加速血液沉降，但其上清液会污染血小板。伴刀豆球蛋白和植物血凝素可加速沉降并获得血浆，但该方法静置1小时才能产生足够量的上清液。由于本研究的目的是开发一种无离心方法，因此将使用钢丝网和磁铁的筛分法应用于乙二胺四乙酸血液、红细胞粘合剂和凝血酶的混合物。该方法在分离血浆方面独具特色，但产量不高，并且在某些测试中化学数据与血清数据不匹配。因此，该试验将是一项新技术，但从技术上判断还需要进一步改进。
