Curey Theodore E, Salazar Mary Alice, Oliveira Philip, Javier James, Dennis Patty J, Rao Poornima, Shear Jason B
Department of Chemistry and Biochemistry, Institute for Cellular & Molecular Biology, Center for Nano and Molecular Science and Technology, University of Texas at Austin, Mail Code A5300, Austin, TX 78712, USA.
Anal Biochem. 2002 Apr 1;303(1):42-8. doi: 10.1006/abio.2001.5551.
An enzyme-based sensor array has been developed to detect multiple disaccharides in aqueous solutions. Porous agarose beads, derivatized with enzymes for assaying disaccharides, are localized within wells etched into a silicon chip in a regular 5 x 7 array. Each well is individually addressable and acts as a microanalysis chamber where sample solution passes through the agarose matrix and is exposed to the enzymes. Detection is achieved by observing the increase in absorbance of a quinoneimine dye produced during the reaction. This technique is used to quantify the disaccharides lactose, sucrose, and maltose and the monosaccharide glucose. Preexisting glucose in the sample complicates multicomponent sensing but can be accounted for by including a glucose sensor in the array. This detection strategy is applied to the simultaneous analysis of these sugars in several beverages.
一种基于酶的传感器阵列已被开发出来，用于检测水溶液中的多种二糖。用用于检测二糖的酶进行衍生化处理的多孔琼脂糖珠，以规则的5×7阵列形式定位在蚀刻于硅芯片中的孔内。每个孔都可单独寻址，并充当微分析室，样品溶液通过琼脂糖基质并与酶接触。通过观察反应过程中产生的醌亚胺染料吸光度的增加来实现检测。该技术用于定量二糖乳糖、蔗糖和麦芽糖以及单糖葡萄糖。样品中预先存在的葡萄糖会使多组分传感变得复杂，但可以通过在阵列中加入葡萄糖传感器来解决。这种检测策略被应用于同时分析几种饮料中的这些糖类。
