Jang A-S, Son M-H
Department of Internal Medicine, Seonam University College of Medicine, Gwangju, Korea.
Allergy. 2002 Apr;57(4):341-5. doi: 10.1034/j.1398-9995.2002.1s3379.x.
The balance between the two subsets of T cell is pivotal for allergic sensitization.
We conducted a cross-sectional study of 486 children vaccinated with bacillus Calmette-Guérin (BCG), aged 10-13 years, to evaluate whether tuberculin responses may contribute to airway hyperresponsiveness (AHR).
Tuberculin skin test, allergic skin test, and methacholine challenge test were done. The methacholine concentration causing a 20% fall (PC20) in forced expiratory volume in 1 second (FEV1) was used as a threshold of AHR. Atopy was defined as a reaction showing a mean wheal size of > or = 3 mm to one or more allergens on skin prick test (SPT). Two tuberculin units of polysorbate-stabilized purified protein derivatives (PPD) were injected intradermally into the volar surface of the forearm. Reactions were read at 48-72 h as the transverse diameter in millimeters of induration.
Of the children in the study, 12.3% (60/486) had PPD induration; 7.8% (38/486) of children had PPD induration of greater than 10 mm. The PPD induration size was 10.5 +/- 1.03 mm (confidence interval (CI) 7.19-12.33) in atopic children and 11.2 +/- 0.76 mm (CI 7.89-13.1) in nonatopic children. The differences of PPD induration diameter between the two groups were not significant. There was no difference of log PC20 between PPD induration > or = 10 mm and < 10 mm (0.13 +/- 0.18 vs. 0.42 +/- 0.05). The difference of log PC20 between positive and negative tuberculin response was not significant. Children with atopy had lower log PC20 than those without atopy (0.16 +/- 0.07 vs. 0.51 +/- 0.05, P = 0.001). After adjusting for sex, age, height, weight, tuberculin response, atopy was associated with AHR in multivariate analyses (odds ratio = 1.895, CI 1.285-2.505, P = 0.002).
These data suggested that a tuberculin response due to mycobacterial infection status have no effect on AHR in schoolchildren.
T细胞两个亚群之间的平衡对于变应性致敏至关重要。
我们对486名接种卡介苗（BCG）的10至13岁儿童进行了一项横断面研究，以评估结核菌素反应是否可能导致气道高反应性（AHR）。
进行了结核菌素皮肤试验、变应性皮肤试验和乙酰甲胆碱激发试验。将导致一秒用力呼气容积（FEV1）下降20%的乙酰甲胆碱浓度（PC20）用作AHR的阈值。特应性定义为皮肤点刺试验（SPT）中对一种或多种变应原的平均风团大小≥3mm的反应。将两个结核菌素单位的聚山梨酯稳定化纯化蛋白衍生物（PPD）皮内注射到前臂掌侧表面。在48至72小时读取反应结果，以硬结的横向直径（毫米）表示。
在研究的儿童中，12.3%（60/486）有PPD硬结；7.8%（38/486）的儿童PPD硬结大于10mm。特应性儿童的PPD硬结大小为10.5±1.03mm（置信区间（CI）7.19 - 12.33），非特应性儿童为11.2±0.76mm（CI 7.89 - 13.1）。两组之间PPD硬结直径的差异不显著。PPD硬结≥10mm和＜10mm之间的log PC20没有差异（0.13±0.18对0.42±0.05）。结核菌素反应阳性和阴性之间的log PC20差异不显著。特应性儿童的log PC20低于非特应性儿童（0.16±0.07对0.51±0.05，P = 0.001）。在对性别、年龄、身高、体重、结核菌素反应进行校正后，多因素分析中特应性与AHR相关（比值比 = 1.895，CI 1.285 - 2.505，P = 0.002）。
这些数据表明，由于分枝杆菌感染状态引起的结核菌素反应对学龄儿童的AHR没有影响。
