Cheng B F, Séguin-Swartz G, Somers D J
Agriculture and Agri-Food Canada, Saskatoon Research Centre, SK.
Genome. 2002 Feb;45(1):110-5. doi: 10.1139/g01-131.
Twenty-two intergeneric hybrids from a cross between Brassica napus (AACC, 2n = 38) cultivar Oro and the ornamental crucifer Orychophragmus violaceus (OO, 2n = 24) were produced without embryo rescue. The plants were classified into three groups based on morphological and cytological observations and RAPD banding patterns. Plants of Group I had morphological traits of both parents and 2n = 29 chromosomes. In these plants, 62.1% of the pollen mother cells (PMCs) had the pairing configuration 1 III + 9 II + 8 I; the remaining PMCs had 10 II + 9 I. The plants possessed 97.6-98.8% B. napus specific and 9.2-11.7% O. violaceus specific RAPD fragments. Plants of Group II exhibited novel morphological traits and possessed 2n = 35, 36, or 37 chromosomes. Plants of Group III were morphologically similar to B. napus and possessed 2n = 19, 37, 38, or 39 chromosomes. Plants of Group II and Group III had 94.1-99.4% B. napus specific RAPD fragments and no O. violaceus specific RAPD fragments. Chromosome fragments were observed in PMCs of most of the F1 plants in all groups. Based on the cytological results and RAPD analysis, it is suggested that genome doubling and chromosome elimination occurred in the intergeneric hybrids of B. napus x O. violaceus.
通过甘蓝型油菜（AACC，2n = 38）品种奥罗与观赏十字花科植物诸葛菜（OO，2n = 24）杂交，在未进行胚挽救的情况下获得了22个属间杂种。根据形态学、细胞学观察以及随机扩增多态性DNA（RAPD）条带模式，将这些植株分为三组。第一组植株具有双亲的形态特征，染色体数为2n = 29。在这些植株中，62.1%的花粉母细胞（PMC）具有1Ⅲ + 9Ⅱ + 8Ⅰ的配对构型；其余的花粉母细胞具有10Ⅱ + 9Ⅰ。这些植株拥有97.6 - 98.8%的甘蓝型油菜特异性RAPD片段和9.2 - 11.7%的诸葛菜特异性RAPD片段。第二组植株表现出新颖的形态特征，染色体数为2n = 35、36或37。第三组植株在形态上与甘蓝型油菜相似，染色体数为2n = 19、37、38或39。第二组和第三组植株具有94.1 - 99.4%的甘蓝型油菜特异性RAPD片段，没有诸葛菜特异性RAPD片段。在所有组的大多数F1植株的花粉母细胞中都观察到了染色体片段。基于细胞学结果和RAPD分析，表明在甘蓝型油菜×诸葛菜的属间杂种中发生了基因组加倍和染色体消除。
