Chubb Jonathan R, Boyle Shelagh, Perry Paul, Bickmore Wendy A
MRC Human Genetics Unit, Western General Hospital, Crewe Road, Edinburgh, EH4 2XU, Scotland, United Kingdom.
Curr Biol. 2002 Mar 19;12(6):439-45. doi: 10.1016/s0960-9822(02)00695-4.
In comparison with many nuclear proteins, the movement of chromatin in nuclei appears to be generally constrained. These restrictions on motion are proposed to reflect the attachment of chromatin to immobile nuclear substructures.
To gain insight into the regulation of chromosome dynamics by nuclear architecture, we have followed the movements of different sites in the human genome in living cells. Here, we show that loci at nucleoli or the nuclear periphery are significantly less mobile than other, more nucleoplasmic loci. Disruption of nucleoli increases the mobility of nucleolar-associated loci.
This is the first report of distinct nuclear substructures constraining the movements of chromatin. These constraints reflect the physical attachment of chromatin to nuclear compartments or steric impairment caused by local ultrastructure. Our data suggest a role for the nucleolus and nuclear periphery in maintaining the three-dimensional organization of chromatin in the human nucleus.
与许多核蛋白相比，染色质在细胞核内的移动似乎普遍受到限制。这些对运动的限制被认为反映了染色质与固定的核亚结构的附着。
为了深入了解核结构对染色体动态的调控，我们追踪了活细胞中人类基因组不同位点的移动。在此，我们表明核仁或核周边的位点移动性明显低于其他更多核质位点。核仁的破坏增加了与核仁相关位点的移动性。
这是关于不同核亚结构限制染色质移动的首次报道。这些限制反映了染色质与核区室的物理附着或由局部超微结构引起的空间阻碍。我们的数据表明核仁及核周边在维持人类细胞核中染色质的三维组织方面发挥作用。
