分子马达：单分子记录变得轻松。

Molecular motors: single-molecule recordings made easy.

作者信息

Diez Stefan, Schief William R, Howard Jonathon

机构信息

Max Planck Institute of Molecular Cell Biology and Genetics, Pfotenhauerstr. 108, 01307 Dresden, Germany.

出版信息

Curr Biol. 2002 Mar 19;12(6):R203-5. doi: 10.1016/s0960-9822(02)00750-9.

DOI:10.1016/s0960-9822(02)00750-9

PMID:11909546

Abstract

A fusion protein of kinesin and gelsolin binds a short actin filament which can be visualized using a standard fluorescence microscope. This technique has provided new insight into the mechanism of kinesin action, and in principle it can be extended to allow single-molecule assays of any protein.

摘要

驱动蛋白和凝溶胶蛋白的融合蛋白结合了一条短肌动蛋白丝，使用标准荧光显微镜即可观察到。这项技术为驱动蛋白的作用机制提供了新的见解，原则上它可以扩展到对任何蛋白质进行单分子分析。