Sun Hyeon Jin, Yoshida Shigeki, Park Nyun Ho, Kusakabe Isao
Institute of Applied Biochemistry, University of Tsukuba, Tsukuba-shi, 1-1-1 Tennoodai, Ibaraki 305-8572, Japan.
Carbohydr Res. 2002 Apr 2;337(7):657-61. doi: 10.1016/s0008-6215(02)00031-9.
Cotton-seed residual cake, which is a byproduct of the process of oil extraction from the seed, was delignified with sodium hypochlorite (1% available chlorine). Xylan was then prepared from the delignified wet material by alkali extraction with 15% sodium hydroxide. The cotton-seed xylan contained 64.7% xylose and 9.4% uronic acid. The xylan was hydrolyzed with 0.125 M sulfuric acid at 90 degrees C for 15 min. The resultant hydrolysis products were separated by gel-permeation chromatography on BioGel P-4 and Toyopearl HW-40F columns connected in series, with water as an eluate. Xylose and xylooligosaccharides with a degree of polymerization ranging from DP 2 to 15 were separated under such conditions, and each xylooligosaccharide-containing peak fraction afforded a single band on fluorophore-assisted carbohydrate electrophoresis. These results suggest that cotton-seed xylan is suitable for the preparation of xylose and xylooligosaccharides.
棉籽残渣饼是棉籽榨油过程中的副产品，用次氯酸钠（有效氯含量1%）进行脱木质素处理。然后用15%的氢氧化钠碱萃取法从脱木质素的湿物料中制备木聚糖。棉籽木聚糖含有64.7%的木糖和9.4%的糖醛酸。将木聚糖在90℃下用0.125 M硫酸水解15分钟。所得水解产物通过串联的BioGel P - 4和Toyopearl HW - 40F柱进行凝胶渗透色谱分离，以水作为洗脱液。在这样的条件下分离出了木糖和聚合度在2到15之间的低聚木糖，并且每个含低聚木糖的峰馏分在荧光辅助碳水化合物电泳中都得到了一条单一的条带。这些结果表明棉籽木聚糖适合用于制备木糖和低聚木糖。
