基础条件下及经X射线处理后人类淋巴细胞中的染色体畸变与谷胱甘肽S-转移酶M1（GSTM1）基因型的关系

Chromosomal aberrations under basal conditions and after treatment with X-ray in human lymphocytes as related to the GSTM1 genotype.

作者信息

Karahalil Bensu, Sardaş Semra, Kocabaş Neslihan Aygün, Alhayiroğlu Ela, Karakaya Ali Esat, Civelek Eren

机构信息

Department of Toxicology, Faculty of Pharmacy, Gazi University, Hipodrom, 06330, Ankara, Turkey.

出版信息

Mutat Res. 2002 Mar 25;515(1-2):135-40. doi: 10.1016/s1383-5718(02)00003-7.

DOI:10.1016/s1383-5718(02)00003-7

PMID:11909761

Abstract

The frequency of chromosomal aberrations (CAs) was evaluated in blood lymphocytes from 18 healthy subjects. Basal CA frequencies were not significantly different in GSTM1 positive and GSTM1 null subjects (P>0.05), whereas they were considerably higher in smokers than in non-smokers. After 1 Gy dose of X-ray challenge of blood samples, CA frequencies were significantly higher in GSTM1 null subjects, compared to GSTM1 positive subjects (P<0.005), and in smokers, compared to non-smokers. These effects are ascribed to the influence of GSTM1 genotype and of smoking status on DNA repair capacities. As the induction of CAs are associated with carcinogenesis, the challenge assay is able to detect enhanced susceptibility for CA caused by genetic predisposition of DNA repair deficiency.

摘要

对18名健康受试者血液淋巴细胞中的染色体畸变（CA）频率进行了评估。谷胱甘肽S-转移酶M1（GSTM1）阳性和GSTM1缺失受试者的基础CA频率无显著差异（P>0.05），而吸烟者的基础CA频率显著高于非吸烟者。对血样进行1 Gy剂量的X射线照射后，与GSTM1阳性受试者相比，GSTM1缺失受试者的CA频率显著更高（P<0.005），与非吸烟者相比，吸烟者的CA频率显著更高。这些影响归因于GSTM1基因型和吸烟状态对DNA修复能力的影响。由于CA的诱导与致癌作用相关，因此该激发试验能够检测出由DNA修复缺陷的遗传易感性导致的CA易感性增强。