Bagger Y Z, Hassager C, Heegaard A M, Christiansen C
Center for Clinical and Basic Research, Ballerup Byvej 222, DK-2750 Ballerup, Denmark.
Climacteric. 2000 Jun;3(2):84-91. doi: 10.3109/13697130009167609.
To investigate the polymorphisms of the vitamin D receptor (VDR) and estrogen receptor (ER) genes in relation to biochemical markers of bone turnover (serum osteocalcin and urinary collagen type I degradation products (CrossLaps), and to study ER genotypes in relation to serum lipoproteins, blood pressure, or changes in these parameters after 2 years of hormone replacement therapy (HRT) in 499 Danish postmenopausal women.
The VDR gene polymorphisms were determined by means of the three restriction enzymes, i.e. BsmI, ApaI and TaqI, while the ER gene polymorphisms were determined by means of the PvuII and XbaI restriction enzymes. Serum osteocalcin, urinary CrossLaps and the lipoproteins were also assessed. Body mass index was recorded.
The VDR or ER genotypes did not differ significantly with respect to age, age at menopause or body mass index. No significant effect of VDR or ER genotype on bone turnover was found. Furthermore, we were unable to find any relationship between ER genotype and lipoproteins or blood pressure at baseline, or changes in these parameters during HRT.
A clinically significant relationship between VDR and ER genotypes and biochemical markers of bone turnover or serum lipoproteins could not be demonstrated in healthy Danish postmenopausal women.
研究维生素D受体（VDR）和雌激素受体（ER）基因多态性与骨转换生化标志物（血清骨钙素和尿I型胶原降解产物（CrossLaps））的关系，并研究499名丹麦绝经后妇女的ER基因型与血清脂蛋白、血压或激素替代疗法（HRT）2年后这些参数变化的关系。
采用BsmI、ApaI和TaqI三种限制性内切酶测定VDR基因多态性，采用PvuII和XbaI限制性内切酶测定ER基因多态性。同时评估血清骨钙素、尿CrossLaps和脂蛋白。记录体重指数。
VDR或ER基因型在年龄、绝经年龄或体重指数方面无显著差异。未发现VDR或ER基因型对骨转换有显著影响。此外，我们未能发现ER基因型与基线时的脂蛋白或血压之间存在任何关系，也未发现HRT期间这些参数的变化。
在健康的丹麦绝经后妇女中，未发现VDR和ER基因型与骨转换生化标志物或血清脂蛋白之间存在具有临床意义的关系。
