Mannechez A, Collet B, Payen L, Lecureur V, Fardel O, Le Moyec L, de Certaines J D, Leray G
LRMBM, Faculté de Médecine, Rennes, France.
Anticancer Res. 2001 Nov-Dec;21(6A):3915-9.
We have previously demonstrated that proton NMR spectra of fatty acid chains in erythroleukemia K562 wild-type cells and their MDR1 counterparts show variations related to the phenotype over-expressing the P-glycoprotein (P-gp). Human lung cancer cells whose multidrug resistance (MDR) counterparts over-express the multidrug resistance-associated protein MRP1 have not yet been studied by NMR. Both P-gp and MRP1 belong to the same ATP-binding cassette transporter superfamily. A comparison of NMR spectra from both these multidrug-resistance phenotypes showed that the results previously obtained on the MDR1 family are not valid for MRP1. Furthermore, flow cytofluorimetry studies with external phosphatidylserine labelling showed that P-gp and MRP1 overexpressions have strong but differentiated effects on cell lipid pools.
我们之前已经证明，红白血病K562野生型细胞及其多药耐药1型（MDR1）对应细胞中脂肪酸链的质子核磁共振（NMR）谱显示出与过表达P-糖蛋白（P-gp）表型相关的变化。多药耐药（MDR）对应细胞过表达多药耐药相关蛋白MRP1的人肺癌细胞尚未通过核磁共振进行研究。P-gp和MRP1都属于同一个ATP结合盒转运蛋白超家族。对这两种多药耐药表型的核磁共振谱进行比较表明，之前在MDR1家族上获得的结果对MRP1无效。此外，使用外部磷脂酰丝氨酸标记的流式细胞荧光测定研究表明，P-gp和MRP1的过表达对细胞脂质池有强烈但有差异的影响。
