用人肺癌细胞系的短期半自动检测法测量辐射反应性。
Radiation responsiveness of human lung cancer cell lines measured with a short term semiautomatic assay.
作者信息
Xing T, Brattstrom D, Bergqvist M, Isaksson U, Wagenius G, Brodin O
机构信息
Department of Oncology, Radiology and Clinical Immunology, Uppsala University Hospital, Sweden.
出版信息
Anticancer Res. 2001 Nov-Dec;21(6A):3925-8.
BACKGROUND
Fluorometric microculture cytotoxicity assay (FMCA) is a short-term semi-automatic method, based on dye-inclusion of surviving cells. The assay was developed for investigations of drug resistance on tumour cells from biopsy material. In the present study, this short-term assay was evaluated, regarding usefulness in determining radio-sensitivity.
MATERIALS AND METHODS
Eight human lung cancer cell lines were used. There were five small cell lung cancer (SCLC and three non-small cell lung cancer (NSCLC cell lines. Results were compared with the corresponding data derived from the clonogenic assay and/or the extrapolation method.
RESULTS
The surviving fraction (SF) after 2, 5 and 10 Gy compared with data from the clonogenic assay were not in accordance for 5 of the 8 cell lines. The FMCA assay overestimated SF- values for the SCLC cell lines.
CONCLUSION
The FMCA assay is not useful as a quick screening method for the radioresponsiveness in vitro of human tumour cell lines.
背景
荧光微量培养细胞毒性测定法（FMCA）是一种基于存活细胞染料掺入的短期半自动方法。该测定法是为研究活检材料中肿瘤细胞的耐药性而开发的。在本研究中，对这种短期测定法在确定放射敏感性方面的实用性进行了评估。
材料与方法
使用了8种人肺癌细胞系。其中有5种小细胞肺癌（SCLC）细胞系和3种非小细胞肺癌（NSCLC）细胞系。将结果与克隆形成测定法和/或外推法得出的相应数据进行比较。
结果
8种细胞系中有5种在2、5和10 Gy照射后的存活分数（SF）与克隆形成测定法的数据不一致。FMCA测定法高估了SCLC细胞系的SF值。
结论
FMCA测定法作为一种快速筛选人肿瘤细胞系体外放射反应性的方法并无用处。
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