Harris D, Engelstein M, Parry R, Smith J, Mabuchi M, Millipore J Leonard
Millipore, Danvers, MA 01923, USA.
Biotechniques. 2002 Mar;32(3):626-8, 630-1. doi: 10.2144/02323pf02.
The accelerating pace of genomics analysis has necessitated the abbreviation of DNA sample preparation protocols. We have developed a size-exclusion-based system for the rapid isolation ofplasmid DNA in a 96-well microplate format. This high-speed protocol employs a modified alkaline lysis methodfor the preparation of the bacterial lysate, followed by three short vacuum filtration steps. Unlike traditional bind/wash/elute methods, there is no need to use chaotropic salts or ethanol. The samples are recovered from the top side of the MultiScreen96 PLASMID plates. Starting with bacterial cell pellets, the entire prycedure for purifying the plasmid DNA can be performed in 30 min with a multichannel pipettor. The high yields, reproducibility, and quality of the plasmids make this system a good choice for any cloning or DNA sequencing operation.
基因组学分析速度的加快使得DNA样品制备方案必须简化。我们开发了一种基于尺寸排阻的系统，用于在96孔微孔板中快速分离质粒DNA。这种高速方案采用改良的碱裂解法制备细菌裂解物，随后进行三个短的真空过滤步骤。与传统的结合/洗涤/洗脱方法不同，无需使用离液盐或乙醇。样品从MultiScreen96 PLASMID板的顶部回收。从细菌细胞沉淀开始，使用多通道移液器可在30分钟内完成纯化质粒DNA的整个过程。质粒的高产量、可重复性和质量使该系统成为任何克隆或DNA测序操作的理想选择。
