Chilton N B, Huby-Chilton F, Gasser R B, Beveridge I
Department of Veterinary Science, University of Melbourne, Parkville, Victoria, 3052, Australia.
Syst Parasitol. 2002 Feb;51(2):81-93. doi: 10.1023/a:1014062830560.
The strongyloid nematode genus Papillostrongylus Johnston & Mawson, 1939, from kangaroos and wallabies, is reviewed using morphological and molecular methods. P. labiatus Johnston & Mawson, 1939 is re-described from material from the type-host, the black-striped wallaby Macropus dorsalis, from eastern Queensland, Australia, in which it is a relatively common parasite. Additional records from M. parryi and Thylogale thetis are confirmed and considered to represent examples of host-switching. A geographically disjunct population of the nematode species occurs in M. bernardus and Petrogale brachyotis in Arnhem Land, Northern Territory, but assessment of its status requires additional material. Nematodes from M. rufus, M. giganteus, M. fuliginosus and M. robustus from inland regions of Australia, formerly attributed to P. labiatus, are here assigned to a new species, P. barbatus, distinguished by the presence of an external leaf-crown, larger size, by greater spicule length in the male and by a sinuous vagina in the female. Additional hosts of P. barbatus n. sp. are Petrogale assimilis and Pet. lateralis purpureicollis. Sequence analyses of the second internal transcribed spacer of ribosomal DNA (ITS-2) also showed that P. barbatus n. sp. differed at 40 (16.7%) of the 240 alignment positions when compared with P. labiatus. Most of these interspecific sequence differences occurred in loops or bulges of the predicted precursor rRNA secondary structure, or represented partial or total compenstory base pair changes in stems.
利用形态学和分子方法，对1939年约翰斯顿和莫森发现的、寄生于袋鼠和沙袋鼠体内的类圆线虫属乳头类圆线虫进行了综述。对来自模式宿主——澳大利亚昆士兰州东部的黑纹沙袋鼠（Macropus dorsalis）的材料进行了重新描述，乳头类圆线虫在该宿主中是一种相对常见的寄生虫。来自帕里沙袋鼠（M. parryi）和蒂氏袋鼬（Thylogale thetis）的其他记录得到了确认，并被认为是宿主转换的例子。在北领地阿纳姆地的伯纳德沙袋鼠（M. bernardus）和短耳岩袋鼠（Petrogale brachyotis）中出现了该线虫物种的地理隔离种群，但对其状况的评估需要更多材料。来自澳大利亚内陆地区的红大袋鼠（M. rufus）、大赤袋鼠（M. giganteus）、烟色大袋鼠（M. fuliginosus）和粗壮袋鼠（M. robustus）体内的线虫，以前被归为唇乳头类圆线虫（P. labiatus），现被归为一个新物种——须乳头类圆线虫（P. barbatus），其特征是有外部叶冠、体型较大、雄性交合刺更长以及雌性阴道呈蜿蜒状。须乳头类圆线虫新物种的其他宿主是似岩袋鼠（Petrogale assimilis）和紫颈岩袋鼠（Pet. lateralis purpureicollis）。核糖体DNA第二内部转录间隔区（ITS-2）的序列分析还表明，与唇乳头类圆线虫相比，须乳头类圆线虫新物种在240个比对位置中有40个（16.7%）不同。这些种间序列差异大多发生在预测的前体rRNA二级结构的环或凸起处，或代表茎中的部分或完全补偿性碱基对变化。
