[黑曲霉在暴露于诱变因素后柠檬酸与草酸比例的变化]
[Change in ratio between citric and oxalic acid in Aspergillus niger upon exposure to mutagenic factors].
作者信息
Golubtsova V M, Shcherbakova E Ia, Runkovskaia L Ia, Ermakova V P
出版信息
Mikrobiologiia. 1979 Nov-Dec;48(6):1060-5.
Stepwise selection of Aspergillus niger producing citric acid in highly buffered molassa media was carried on in order to decrease the biosynthesis of oxalic and gluconic acids using the following mutagenic factors: diethyl sulfate, thiophosphamide, cyclophosphan, N-methyl-N'-nitro-N-nitrosoguanidine (NG), 5-fluorouracil, 1,4-bis-diazoacetylbutane, and UV. The ratio between the acids changed in mutants obtained upon the combined treatment with UV and NG. Mutants producing twice as less oxalic acid were characterized by weak growth and formation of conidia.
为了减少草酸和葡萄糖酸的生物合成，利用以下诱变因素（硫酸二乙酯、硫磷酰胺、环磷酰胺、N-甲基-N'-硝基-N-亚硝基胍（NG）、5-氟尿嘧啶、1,4-双重氮乙酰丁烷和紫外线），在高度缓冲的糖蜜培养基中对产柠檬酸的黑曲霉进行逐步筛选。在用紫外线和NG联合处理后获得的突变体中，酸之间的比例发生了变化。草酸产量减少两倍的突变体的特征是生长微弱和分生孢子形成。
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