Hisatomi Hisashi, Nagao Kumi, Wakita Kazuyuki, Kohno Norio
Center for Molecular Biology and Cytogenetics, SRL, Inc., Tokyo, Japan.
Oncology. 2002;62(2):136-40. doi: 10.1159/000048259.
Allelic loss frequently occurs on the short arm of chromosome 1 in human breast carcinoma, suggesting that the ARHI/NOEY2 gene, an imprinted putative tumor suppressor gene, is involved in the pathogenesis of the tumor entity. To clarify the clinical importance of ARHI/NOEY2 mRNA in breast cancer, we studied whether ARHI/NOEY2 inactivation might contribute to tumors arising in the breast. An ARHI/NOEY2 message was detected by real-time PCR analysis in all noncancerous breast tissues, but was not detected in 2 of 26 breast cancer tissue samples. In 10 of 26 breast cancer tissue samples ARHI/NOEY2 mRNA was substantially reduced. ARHI/NOEY2 expression was lost or markedly reduced in 12 of 26 (46.15%) breast cancer tissue samples. In summary, we conclude that decreased ARHI/NOEY2 mRNA expression may play an important role in the pathogenesis of breast cancer.
等位基因缺失在人类乳腺癌中经常发生在1号染色体短臂上，这表明ARHI/NOEY2基因，一个印记的假定肿瘤抑制基因，参与了该肿瘤实体的发病机制。为了阐明ARHI/NOEY2 mRNA在乳腺癌中的临床重要性，我们研究了ARHI/NOEY2失活是否可能导致乳腺肿瘤的发生。通过实时PCR分析在所有非癌性乳腺组织中检测到ARHI/NOEY2信息，但在26个乳腺癌组织样本中的2个中未检测到。在26个乳腺癌组织样本中的10个中，ARHI/NOEY2 mRNA大幅减少。在26个（46.15%）乳腺癌组织样本中的12个中，ARHI/NOEY2表达缺失或显著降低。总之，我们得出结论，ARHI/NOEY2 mRNA表达降低可能在乳腺癌发病机制中起重要作用。
