Lehoczki E
Biochim Biophys Acta. 1975 Dec 11;408(3):223-7. doi: 10.1016/0005-2728(75)90125-5.
The absorption spectra of chlorophyll b in Triton X-100 micelles at room temperature are superpositions of components with absorption maxima at 640.8, 648.9, 659.5, 669.6, 682.1 and 695.7 nm, obtained from Gaussian analysis of the spectra. The last four forms strongly overlap the chlorophyll a forms of this system obtained with maxima at 659.3, 667.6, 674.3, 680.8, 686.5, 692.8, 701.9, 713.6 and 722.0 nm. Since the in vivo chlorophyll a forms practically coincide with the forms found in this system, the possible existence of in vivo overlapping chlorophyll b and a forms eventually should be taken into consideration. In this case, however, the Gaussian analysis of in vivo absorption bands in itself in the proper spectrum range cannot discriminate between chlorophyll a and b components.
叶绿素b在室温下于Triton X - 100胶束中的吸收光谱是由光谱的高斯分析得到的、吸收峰分别位于640.8、648.9、659.5、669.6、682.1和695.7 nm的各组分的叠加。最后四种形式与该体系中叶绿素a的形式强烈重叠，叶绿素a的吸收峰位于659.3、667.6、674.3、680.8、686.5、692.8、701.9、713.6和722.0 nm。由于体内叶绿素a的形式实际上与该体系中发现的形式一致，最终应考虑体内叶绿素b和a形式可能存在重叠的情况。然而，在这种情况下，在适当光谱范围内对体内吸收带进行高斯分析本身无法区分叶绿素a和b组分。
