Machovich R
Biochim Biophys Acta. 1975 Nov 18;412(1):13-7. doi: 10.1016/0005-2795(75)90334-7.
It has been suggested that heparin can affect blood coagulation through thrombin, i.e. the binding of heparin to thrombin induces a conformational change in the enzyme, facilitating a complex formation between thrombin and antithrombin (Machovich, T., Blaskó, Gy. and Pálos, L. (1975) Biochim. Biophys. Acta 379, 193-200). This hypothesis seems to have been proved. Modification of arginine residues in thrombin did not result in decreased thrombin activity and decreased sensitivity to antithrombin, whereas the heparin sensitivity of the enzyme and the thrombin-antithrombin reaction were diminished.
有人提出，肝素可通过凝血酶影响血液凝固，即肝素与凝血酶的结合会诱导该酶发生构象变化，促进凝血酶与抗凝血酶之间形成复合物（马乔维奇，T.，布拉斯科，Gy.和帕洛斯，L.（1975年）《生物化学与生物物理学报》379卷，第193 - 200页）。这一假说似乎已得到证实。凝血酶中精氨酸残基的修饰并未导致凝血酶活性降低以及对抗凝血酶敏感性降低，而该酶对肝素的敏感性和凝血酶 - 抗凝血酶反应却减弱了。
