Rosengren J, Pählman S, Glad M, Hjertén S
Biochim Biophys Acta. 1975 Nov 18;412(1):51-61.
A series of agarose gels, substituted with hydrophobic groups, has been synthesized and used for binding studies with the coloured model protein, phycoerythrin. The degrees of substitution for the derivatives can easily be estimated with proton magnetic resonance (NMR). It has been found that the capacity of the derivatives for phycoerythrin increases with increasing hydrophobicity of the substituent, degree of substitution and increasing ionic strength. For column experiments the degree of substitution should lie in the range 40-100 mmol substituent/mol galactose. When it is excessively high, the flow characteristics of the columns are unsatisfactory and difficulties to achieve complete desorption may arise.
一系列带有疏水基团的琼脂糖凝胶已被合成，并用于与有色模型蛋白藻红蛋白的结合研究。衍生物的取代度可以很容易地用质子磁共振（NMR）来估计。已经发现，衍生物对藻红蛋白的结合能力随着取代基疏水性的增加、取代度的增加和离子强度的增加而增加。对于柱实验，取代度应在40 - 100 mmol取代基/摩尔半乳糖的范围内。当取代度过高时，柱的流动特性不理想，可能会出现难以实现完全解吸的情况。
