Sairam M R, Li C H
Biochim Biophys Acta. 1975 Nov 18;412(1):70-81.
The separation of alpha and beta subunits from human pituitary lutropin is described, and the complete amino acid sequence of the beta subunit is proposed. It consists of 115 amino acids with serine and glycine at the amino and carboxyl termini, respectively. The single carbohydrate moiety is linked to asparagine in position 30 and the single tryptophan of the lutropin molecule is located at position 8. The two methionine residues in lutropin-beta are in positions 41 and 42. In addition to COOH-terminal heterogeneity, evidence for internal peptide cleavages was observed.
本文描述了从人垂体促黄体生成素中分离出α和β亚基的过程，并提出了β亚基的完整氨基酸序列。它由115个氨基酸组成，氨基末端和羧基末端分别为丝氨酸和甘氨酸。单个糖基部分连接在第30位的天冬酰胺上，促黄体生成素分子中的单个色氨酸位于第8位。促黄体生成素β中的两个甲硫氨酸残基分别位于第41位和第42位。除了羧基末端的异质性外，还观察到内部肽段裂解的证据。
