从人初乳乳清中分离和鉴定几种糖蛋白。

Isolation and characterization of several glycoproteins from human colostrum whey.

作者信息

Nichols J H, Bezkorovainy A, Paque R

出版信息

Biochim Biophys Acta. 1975 Nov 18;412(1):99-108. doi: 10.1016/0005-2795(75)90343-8.

PMID:1191679

Abstract

Five homogeneous closely related glycoproteins were isolated from human colostrum whey, and their molecular weights and carbohydrate contents ranged from 26 000 to 35 000 and from 50 to 80%, respectively. All had the Lactobacillus bifidus var. Pennsylvanicus growth-promoting activity, which was not substantially altered by desialization. Neither human colostrum, milk or the glycoproteins had any cellular immunity-mediating activity. Degradation of the carbohydrate moieties of these glycoproteins indicated that each contained non-identical carbohydrate chains.

摘要

从人初乳乳清中分离出了五种同源且密切相关的糖蛋白，它们的分子量在26000至35000之间，碳水化合物含量分别在50%至80%之间。所有这些糖蛋白都具有促进两歧双歧杆菌宾夕法尼亚变种生长的活性，去唾液酸化处理后该活性基本未变。人初乳、乳汁或这些糖蛋白均无任何细胞免疫介导活性。这些糖蛋白碳水化合物部分的降解表明，每种糖蛋白都含有不同的碳水化合物链。