Schrayer David P, Kouttab Nicola, Hearing Vincent J, Wanebo Harold J
Department of Surgery and Pathology, University Medical Group/Roger Williams Medical Center, Boston University School of Medicine, Providence, Rhode Island 02908, USA.
Clin Exp Metastasis. 2002;19(1):43-53. doi: 10.1023/a:1013875104326.
We have previously reported that immunization of mice with melanoma cells transfected to secrete the superantigen, Staphylococcal enterotoxin A (SEA), increased the production of antibodies to the B700 melanoma antigen, stimulated the production of endogenous interleukin 2 (IL-2), activated the expression of CD4, CD8 and CD25 T cell markers and enhanced NK cell activity. Now we show that immunization of mice with a vaccine of irradiated sea-transfected melanoma cells coupled with IL-2 therapy was even more effective in inhibiting the growth of primary melanoma tumors and the development of lung metastases than was the irradiated melanoma cell vaccine alone or IL-2 alone. The morphological and immunological effectiveness of the therapy was dose-dependent on IL-2.
我们先前曾报道，用转染后分泌超抗原葡萄球菌肠毒素A（SEA）的黑色素瘤细胞免疫小鼠，可增加针对B700黑色素瘤抗原的抗体产生，刺激内源性白细胞介素2（IL-2）的产生，激活CD4、CD8和CD25 T细胞标志物的表达，并增强自然杀伤细胞（NK）活性。现在我们发现，用经照射的转染SEA的黑色素瘤细胞疫苗联合IL-2治疗免疫小鼠，在抑制原发性黑色素瘤肿瘤生长和肺转移发生方面，比单独使用经照射的黑色素瘤细胞疫苗或单独使用IL-2更为有效。该治疗的形态学和免疫学效果在IL-2方面呈剂量依赖性。
