Tsai Wei-Bor, Grunkemeier John M, McFarland Clive D, Horbett Thomas A
Department of Bioengineering, University of Washington, Seattle, Washington 98195, USA.
J Biomed Mater Res. 2002 Jun 5;60(3):348-59. doi: 10.1002/jbm.10048.
Four plasma proteins have been shown to be able to mediate platelet adhesion to synthetic materials when they are adsorbed as purified proteins: fibrinogen (Fg), fibronectin (Fn), vitronectin (Vn), and von Willebrand factor (vWF). Among them, Fg is thought to play a leading role in mediating platelet adhesion to plasma-preadsorbed biomaterials, but this has been established for only three types of materials so far in our laboratory. Furthermore, the role of Fn, Vn, and vWF in mediating platelet adhesion to plasma-preadsorbed surfaces is still unclear. The aim of the current study was to assess the importance of Fg, Fn, Vn, and vWF in mediating platelet adhesion to a series of polystyrene-based surfaces. The strategy applied in the present investigation was to compare platelet adhesion to surfaces preadsorbed with normal plasma, plasma selectively depleted in Fn or Vn or both Fn and Vn, plasma from donors who were genetically deficient in vWF, and serum. Few platelets adhered to the surfaces preadsorbed with serum, whereas depletion of Fn, Vn, or vWF from plasma did not decrease platelet adhesion significantly. Replenishment of exogenous Fg to serum before protein adsorption restored platelet adhesion to the surfaces, suggesting that Fg was the major plasma protein that mediated platelet adhesion. Also, we found that a surface density of adsorbed Fg far below the amount that usually adsorbs to synthetic surfaces was sufficient to support full-scale platelet adhesion.
已有研究表明，四种血浆蛋白作为纯化蛋白被吸附时，能够介导血小板与合成材料的黏附：纤维蛋白原（Fg）、纤连蛋白（Fn）、玻连蛋白（Vn）和血管性血友病因子（vWF）。其中，Fg被认为在介导血小板与血浆预吸附生物材料的黏附中起主导作用，但到目前为止，在我们实验室中仅针对三种材料证实了这一点。此外，Fn、Vn和vWF在介导血小板与血浆预吸附表面黏附中的作用仍不清楚。本研究的目的是评估Fg、Fn、Vn和vWF在介导血小板与一系列聚苯乙烯基表面黏附中的重要性。本研究采用的策略是比较血小板与预先吸附有正常血浆、选择性缺乏Fn或Vn或同时缺乏Fn和Vn的血浆、vWF基因缺陷供体的血浆以及血清的表面的黏附情况。很少有血小板黏附于预先吸附有血清的表面，而从血浆中去除Fn、Vn或vWF并不会显著降低血小板黏附。在蛋白质吸附前向血清中补充外源性Fg可恢复血小板与表面的黏附，这表明Fg是介导血小板黏附的主要血浆蛋白。此外，我们发现，吸附的Fg的表面密度远低于通常吸附到合成表面的量，就足以支持全面的血小板黏附。
