Zapata Rafael, Martín David, Piulachs Maria-Dolors, Bellés Xavier
Department of Physiology and Molecular Biodiversity, Institut de Biologia Molecular de Barcelona, Barcelona, Spain.
Arch Insect Biochem Physiol. 2002 Apr;49(4):177-86. doi: 10.1002/arch.10018.
In the fat body of adult Blattella germanica females, the expression of 3-hydroxy-3-methylglutaryl-CoA reductase (HMG-CoA reductase) during the first reproductive cycle is parallel to that of vitellogenin, suggesting a functional link between the mevalonate pathway, and vitellogenesis and reproduction. We have studied the effects of compactin and fluvastatin, two inhibitors of HMG-CoA reductase, on the expression and activity of the enzyme in the fat body, and on the ootheca formation, ootheca viability, and number of larvae per viable ootheca. Short-term assays showed that both compounds reduce the protein levels and enzymatic activity of HMG-CoA reductase, and long-term experiments revealed that fluvastatin impairs embryo development.
在成年德国小蠊雌性的脂肪体中，3-羟基-3-甲基戊二酰辅酶A还原酶（HMG-CoA还原酶）在第一个生殖周期中的表达与卵黄原蛋白的表达平行，这表明甲羟戊酸途径与卵黄发生和繁殖之间存在功能联系。我们研究了两种HMG-CoA还原酶抑制剂——洛伐他汀和氟伐他汀，对脂肪体中该酶的表达和活性以及卵鞘形成、卵鞘活力和每个存活卵鞘中幼虫数量的影响。短期试验表明，这两种化合物均降低了HMG-CoA还原酶的蛋白质水平和酶活性，长期实验显示氟伐他汀会损害胚胎发育。
