Matee M I, Matre R
Department of Microbiology and Immunology, Faculty of Medicine, Muhimbili University College of Health Sciences, P.O. Box 65001, Dar es Salaam, Tanzania.
East Afr Med J. 2001 Sep;78(9):458-60. doi: 10.4314/eamj.v78i9.8974.
To determine, from laboratory records, the spectrum of bacterial and fungal pathogens isolated from cerebrospinal fluids (CSF) of in-patients with meningitis at Muhimbili Medical Centre (MMC) in Dar es Salaam and to ascertain the laboratory results (based on microscopy and culture) using the latex agglutination technique.
A retrospective study based on laboratory records of CSF samples investigated between November 1999 and June 2000 and a cross-sectional study involving investigation of 60 freshly collected CSF samples by conventional (microscopy and culture) and antigen detection by latex agglutination technique (LAT).
Muhimbili Medical Centre in Dar es Salaam, Tanzania.
Information from laboratory records, bacteriological examination of CSF by microscopy, culture and agglutination techniques.
According to records, a total of 1144 CSF samples were investigated between November 1999 and June 2000, of which two hundred and twenty two specimens (19.4%) had a positive bacterial or fungal culture. Fifty five of the isolates were from children (aged less than 15 years) and were; 20 (36.4%) were Streptococcus pneumoniae, 12(21.8%) were Cryptococcus neoformans, and nine (16.4%) were Haemophilus influenzae type b. The remaining 14 (25%) isolates included three group B streptococci, three Klebsiella spp, two E. coli, two Staphylococcus aureus, two Pseudomonas spp, one Moraxella and one Salmonella group B. For adults a total of 167 positive cultures were reported and 163 (97.6%) of the isolates were Cryptococcus neoformans, two (1.2%) were Pseudomonas spp. and two were S. aureus. There was good agreement between conventional microscopy and culture with the latex agglutination technique in the identification of CSF pathogens.
In children, S. pneumonia, and bacteria in general constituted the majority of isolates. Adult cases of meningitis were almost exclusively due to C. neoformans. Overall, C. neoformans appears to be the most common isolate among meningitis cases. Based on LAT results, our routine diagnostic methods seem to be adequate in the identification of the common CSF pathogens.
根据实验室记录，确定在达累斯萨拉姆的穆希姆比利医疗中心（MMC）住院的脑膜炎患者脑脊液（CSF）中分离出的细菌和真菌病原体谱，并使用乳胶凝集技术确定实验室结果（基于显微镜检查和培养）。
一项基于1999年11月至2000年6月期间对脑脊液样本实验室记录的回顾性研究，以及一项横断面研究，该研究涉及通过常规方法（显微镜检查和培养）对60份新采集的脑脊液样本进行调查，并通过乳胶凝集技术（LAT）进行抗原检测。
坦桑尼亚达累斯萨拉姆的穆希姆比利医疗中心。
来自实验室记录的信息、通过显微镜检查、培养和凝集技术对脑脊液进行细菌学检查。
根据记录，1999年11月至2000年6月期间共调查了1144份脑脊液样本，其中222份标本（19.4%）细菌或真菌培养呈阳性。55株分离菌来自儿童（年龄小于15岁），其中：20株（36.4%）为肺炎链球菌，12株（21.8%）为新型隐球菌，9株（16.4%）为b型流感嗜血杆菌。其余14株（25%）分离菌包括3株B组链球菌、3株克雷伯菌属、2株大肠杆菌、2株金黄色葡萄球菌、2株假单胞菌属、1株莫拉菌属和1株B组沙门菌。对于成年人，共报告了167份阳性培养物，其中163株（97.6%）分离菌为新型隐球菌，2株（1.2%）为假单胞菌属，2株为金黄色葡萄球菌。在脑脊液病原体鉴定方面，常规显微镜检查和培养与乳胶凝集技术之间具有良好的一致性。
在儿童中，肺炎链球菌和一般细菌构成了分离菌的大多数。成人脑膜炎病例几乎完全由新型隐球菌引起。总体而言，新型隐球菌似乎是脑膜炎病例中最常见的分离菌。基于乳胶凝集试验结果，我们的常规诊断方法似乎足以鉴定常见的脑脊液病原体。
