Raghu Anekal R, Nirmala N Rao, Sreekumaran Nair
Department of Oral Pathology and Microbiology, College of Dental Surgery, Manipal, Karnataka, India.
Quintessence Int. 2002 Mar;33(3):234-9.
To aid in differentiation of the two lesions, direct immunofluorescence was used to examine the fluorescence patterns in oral lichen planus and oral lichenoid reactions and to compare the degree of intensity of their fluorescence.
Thirty patients participated in this study. Oral mucosal biopsy specimens were obtained from the edge of fresh lesions and were hemisected. One of the sections was placed in 10% neutral buffered formalin for standard light microscopic examination and the other was washed in phosphate-buffered saline and transferred to Michel's transport media for direct immunofluorescence examination.
Fibrinogen was deposited in specimens from 14 of 15 patients diagnosed with oral lichen planus; lesions showed an intense positive fluorescence that outlined the basement membrane zone. Eight of 10 sections from oral lichenoid reactions exhibited fibrinogen deposition along the basement membrane zone; the specimens showed a less intense, homogeneous linear fluorescence. Specimens from five control samples showed no fluorescence.
Fibrinogen was deposited at the basement membrane zone in both oral lichen planus and suspected oral lichenoid reactions, but the fluorescence was less intense in the oral lichenoid lesions.
为辅助鉴别这两种病变，采用直接免疫荧光法检查口腔扁平苔藓和口腔苔藓样反应中的荧光模式，并比较其荧光强度。
30名患者参与了本研究。从新鲜病变边缘获取口腔黏膜活检标本并将其对半切开。其中一部分切片置于10%中性缓冲福尔马林中用于标准光学显微镜检查，另一部分在磷酸盐缓冲盐水中冲洗后转移至米歇尔运输培养基中用于直接免疫荧光检查。
在15例诊断为口腔扁平苔藓的患者中，有14例标本出现纤维蛋白原沉积；病变显示出强烈的阳性荧光，勾勒出基底膜带。10例口腔苔藓样反应切片中有8例沿基底膜带出现纤维蛋白原沉积；标本显示荧光强度较弱、呈均匀的线性荧光。5份对照样本的标本未显示荧光。
口腔扁平苔藓和疑似口腔苔藓样反应中均在基底膜带出现纤维蛋白原沉积，但口腔苔藓样病变中的荧光较弱。
