Navaratnam V, Lewis P R
Brain Res. 1975 Dec 26;100(3):599-613. doi: 10.1016/0006-8993(75)90160-2.
Histochemical study of the distribution of cholinesterases in the cat medulla oblongata reveals that all neurones in the dorsal motor nucleus of the vagus (DMV) contain true cholinesterase (AChE) but, while most contain this enzyme alone, a small proportion of cells contain pseudocholinesterase (BuChE) as well. Cervical vagotomy affects the two types of cell to different degrees of severity. The BuChE-containing neurones lose their enzyme completely within 2-3 weeks and they atrophy and disappear as a result of the operation. On the other hand, the reaction is less severe and is reversible in those cells containing AChE only. Vagotomy also causes reduction of AChE and BuChE staining in the nearby area subpostrema; the depletion here is pronounced at 2-3 weeks and recovery occurs within the year. These findings suggest that some cells in the area subpostrema project peripherally via the vagus and that the area is part of the vagal nuclear complex. Moreover, capilllaries in the area contain AChE and BuChE in the endothelial lining and this is one of the few areas of the cat hindbrain to exhibit such vascular enzyme activity. The ependyma of the area postrema, which overlies the area subpostrema, is heavily stained for BuChE but this is unaffected by vagotomy.
对猫延髓中胆碱酯酶分布的组织化学研究表明，迷走神经背运动核（DMV）中的所有神经元都含有真性胆碱酯酶（AChE），但大多数神经元仅含有这种酶，一小部分细胞还含有假性胆碱酯酶（BuChE）。颈迷走神经切断术对这两种类型的细胞产生不同程度的严重影响。含BuChE的神经元在2至3周内完全失去其酶，并且由于手术而萎缩并消失。另一方面，仅含AChE的细胞反应较轻且可逆。迷走神经切断术还会导致最后区附近区域的AChE和BuChE染色减少；此处的消耗在2至3周时明显，一年内恢复。这些发现表明，最后区的一些细胞通过迷走神经向周围投射，并且该区域是迷走神经核复合体的一部分。此外，该区域的毛细血管在内皮衬里含有AChE和BuChE，这是猫后脑少数表现出这种血管酶活性的区域之一。最后区上方的最后区室管膜对BuChE染色很深，但这不受迷走神经切断术的影响。
