Ali M, Capindale J B
Can J Biochem. 1975 Nov;53(11):1138-44.
The release of ammonia by hydrolysis of N-benzoyl-L-asparagine, glycyl-DL-asparagine, L-asparagine, and succinamic acid, and of aniline from N-benzoyl-L-glutamic-alpha-anilide, N-benzoyl-L-aspartic-alpha-anilide, L-aspartic-alpha-anilide, and the monoanilides of succinic and glutaric acids is first-order with respect to substrate in dilute (0.4-0.03 M) aqueous hydrochloric acid at 100 degrees C. The first-order rate constants (kobs) for these reactions can be expressed as kobs = kintra + k2[H+]. The above hydrolyses are used as models for developing a tentative mechanism to account for the selective release of aspartic acid from proteins under these condiditons. The data are also used to suggest reasons why glutamic acid is not released with equal facility.
在100℃的稀（0.4 - 0.03M）盐酸水溶液中，N - 苯甲酰 - L - 天冬酰胺、甘氨酰 - DL - 天冬酰胺、L - 天冬酰胺和琥珀酰胺酸水解产生氨，以及N - 苯甲酰 - L - 谷氨酸 - α - 苯胺、N - 苯甲酰 - L - 天冬氨酸 - α - 苯胺、L - 天冬氨酸 - α - 苯胺以及琥珀酸和戊二酸的单苯胺水解产生苯胺的反应，对底物而言是一级反应。这些反应的一级速率常数（kobs）可表示为kobs = kintra + k2[H⁺]。上述水解反应被用作模型，以建立一个初步的机制，来解释在这些条件下蛋白质中天冬氨酸的选择性释放。这些数据还被用于说明谷氨酸没有以相同程度释放的原因。
