Issaq Haleem J, Veenstra Timothy D, Conrads Thomas P, Felschow Donna
SAIC-Frederick, Inc., National Cancer Institute at Frederick, National Institutes of Health, Frederick, Maryland, USA.
Biochem Biophys Res Commun. 2002 Apr 5;292(3):587-92. doi: 10.1006/bbrc.2002.6678.
The need for methods to identify disease biomarkers is underscored by the survival-rate of patients diagnosed at early stages of cancer progression. Surface enhanced laser desorption/ionization time-of-flight mass spectrometry (SELDI-TOF MS) is a novel approach to biomarker discovery that combines two powerful techniques: chromatography and mass spectrometry. One of the key features of SELDI-TOF MS is its ability to provide a rapid protein expression profile from a variety of biological and clinical samples. It has been used for biomarker identification as well as the study of protein-protein, and protein-DNA interaction. The versatility of SELDI-TOF MS has allowed its use in projects ranging from the identification of potential diagnostic markers for prostate, bladder, breast, and ovarian cancers and Alzheimer's disease, to the study of biomolecular interactions and the characterization of posttranslational modifications. In this minireview we discuss the application of SELDI-TOF MS to protein biomarker discovery and profiling.
癌症进展早期诊断患者的生存率凸显了识别疾病生物标志物方法的必要性。表面增强激光解吸/电离飞行时间质谱（SELDI-TOF MS）是一种发现生物标志物的新方法，它结合了两种强大的技术：色谱法和质谱法。SELDI-TOF MS的关键特性之一是能够从各种生物和临床样本中快速提供蛋白质表达谱。它已被用于生物标志物的识别以及蛋白质-蛋白质和蛋白质-DNA相互作用的研究。SELDI-TOF MS的多功能性使其可用于从识别前列腺癌、膀胱癌、乳腺癌、卵巢癌和阿尔茨海默病的潜在诊断标志物到生物分子相互作用研究和翻译后修饰表征等各种项目。在本综述中，我们讨论SELDI-TOF MS在蛋白质生物标志物发现和分析中的应用。
