[Cloning of the human cytochrome p450 2B6 gene in Escherichia coli cells].
作者信息
Kolomeĭchuk S N, Pokrovskaia M V, Borisova A A, Zhgun A A, Solodar' L I, Sokolov N N, Shvets V I, Gervaziev Iu V, El'darov M A, Archakov A I
机构信息
Orekhovich Institute of Biomedical Chemistry RAMS, Pogodinskaya 10, Moscow 119992.
出版信息
Vopr Med Khim. 2001 Nov-Dec;47(6):609-19.
PMID:11925752
Abstract
Human cytochrome P450 2B6 gene from plasmid pUC9, carrying cytochrome CYP2B6 cDNA was cloned into eucariotic expression vector pcDNA 3.1 (+). Cytochrome P450 2B6 gene in recombinant plasmid pcDNA 3.1 (+)/CYP 2B6 was expressed in E. coli cells. The expression of catalytically active recombinant protein was 60-100 nm per liter of the culture medium.
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