Kolomeĭchuk S N, Pokrovskaia M V, Borisova A A, Zhgun A A, Solodar' L I, Sokolov N N, Shvets V I, Gervaziev Iu V, El'darov M A, Archakov A I
Orekhovich Institute of Biomedical Chemistry RAMS, Pogodinskaya 10, Moscow 119992.
Vopr Med Khim. 2001 Nov-Dec;47(6):609-19.
Human cytochrome P450 2B6 gene from plasmid pUC9, carrying cytochrome CYP2B6 cDNA was cloned into eucariotic expression vector pcDNA 3.1 (+). Cytochrome P450 2B6 gene in recombinant plasmid pcDNA 3.1 (+)/CYP 2B6 was expressed in E. coli cells. The expression of catalytically active recombinant protein was 60-100 nm per liter of the culture medium.
