Ohi S, Hosaka K, Ohkawa M, Sato K
Department of Applied Biological Science, Nihon University.
Hum Cell. 2001 Dec;14(4):317-22.
We examined whether metaphase nuclei could be used as nuclear donors in nuclear transfer in mice. The reconstructed embryos were developed to fetuses in both the metaphase-nuclear transfer and the G1-nuclear transfer. We also performed enucleation of oocytes following nuclear injection (injection-enucleation method) using microinjection method with a piezo-driven micromanipulator in order to produce the cloned murine fetuses. We found that this method could shorten time for manipulation in comparison with the conventional method performing nuclear injection following enucleation of oocytes (enucleation-injection method). We produced successfully cloned fetuses by the injection-enucleation method. Furthermore, there was no difference of developmental efficiency in reconstructed embryos from between B6D2F1 and ddY strain as oocyte donor.
我们研究了中期细胞核是否可用作小鼠核移植的核供体。中期核移植和G1期核移植的重构胚胎均发育成了胎儿。我们还使用压电驱动显微操作器的显微注射方法在核注射后进行卵母细胞去核（注射-去核法），以产生克隆的小鼠胎儿。我们发现，与卵母细胞去核后进行核注射的传统方法（去核-注射法）相比，该方法可缩短操作时间。我们通过注射-去核法成功产生了克隆胎儿。此外，以B6D2F1和ddY品系作为卵母细胞供体时，重构胚胎的发育效率没有差异。
