Yoshimoto Koji, Iwaki Toru, Inamura Takanori, Fukui Masashi, Tahira Tomoko, Hayashi Kenshi
Division of Genome Analysis, Research Center for Genetic Information, Medical Institute of Bioregulation, Kyushu University, Higashi-ku, Fukuoka 812-8582, Japan.
Jpn J Cancer Res. 2002 Mar;93(3):284-90. doi: 10.1111/j.1349-7006.2002.tb02170.x.
Detection of the loss of chromosomal regions in cancerous tissues has diagnostic and prognostic relevance, and the development of a reliable and cost-effective technique for this is clinically important. Here we present an efficient technique for quantitative detection of microsatellite alleles, using a post-PCR fluorescence-labeling procedure and multiplexed analysis. We also present a new statistical method for the interpretation of the data that permits reliable and sensitive evaluation of the allelic status of sampled DNA. A high-resolution analysis of allelic imbalance on chromosomes 1p, 10 and 19q in 28 glioma samples of various types using this method revealed that allelic imbalances are more frequent than have been reported, suggesting the diagnostic value of this method in examining the genetic profiles of gliomas.
检测癌组织中染色体区域的缺失具有诊断和预后意义，为此开发一种可靠且经济高效的技术在临床上具有重要意义。在此，我们介绍一种高效的微卫星等位基因定量检测技术，该技术采用PCR后荧光标记程序和多重分析。我们还提出了一种新的统计方法来解释数据，该方法能够对采样DNA的等位基因状态进行可靠且灵敏的评估。使用该方法对28个不同类型的神经胶质瘤样本中的1号染色体短臂、10号染色体和19号染色体长臂上的等位基因不平衡进行高分辨率分析，结果显示等位基因不平衡比之前报道的更为频繁，这表明该方法在检测神经胶质瘤基因图谱方面具有诊断价值。
