Ji Y, Guan H, Gu L
First Affiliated Hospital of Dalian Medical University, Dalian 116011, China.
Zhonghua Jie He He Hu Xi Za Zhi. 2001 Dec;24(12):718-21.
To study the significance of thrombosis after experimental pulmonary thromboembolism (PTE).
Acute PTE models of rabbits were established with injection of autologous blood clots (0.04 g/kg) stabilized in a temperature-controlled (70 degrees C) of distilled water for 10 minutes through the femoral vein, then the regulation of thrombosis was explored at dissection and upon microscopic examination after PTE. Moreover, the coagulability of blood and the plasma level of thromboxane A2(TXA2) and endothelin (ET) were examined.
Thrombotic propensity was found at 1 h, and fresh thrombosis started to form at 24 h following clots infusion. Emboli were completely or partly dissolved at 5 d and organized at 10 and 14 d after clots infused. Prothrombin time was significantly lower [(7.15 +/- 0.06)s], and fibrinogen was higher [(5.86 +/- 1.50) g/L] at 24 h post-clots, compared with pre-clots [(7.34 +/- 0.19)s, (3.37 +/- 1.02) g/L] (P < 0.05). Venous plasma level of TXA2 began to increase at 5 min [(2.5 +/- 0.7) micrograms/L] and continued to rise to its maximum at 15 min [(2.5 +/- 0.6) micrograms/L], then declined at 60 min after clots infusion. The level of ET in both arterial and venous blood increased at 5 d post-clots [(0.84 +/- 0.15) micrograms/L and (0.23 +/- 0.05) micrograms/L] separately, while most of emboli resolved.
There is thrombus formation after autologous-blood-clots-induced PTE. Furthermore, thrombus formation, fibrinolysis and organization may always interact on each other consistently, and control the pathogenesis of PTE. Abnormalities of ET metabolism occur after PTE and the major mediator of TXA2 plays an important role in the early phase of PTE.
研究实验性肺血栓栓塞症（PTE）后血栓形成的意义。
通过股静脉注射在70℃蒸馏水中稳定10分钟的自体血凝块（0.04 g/kg）建立兔急性PTE模型，然后在PTE后解剖及显微镜检查时探讨血栓形成的规律。此外，检测血液凝固性及血栓素A2（TXA2）和内皮素（ET）的血浆水平。
注入血凝块后1小时发现有血栓形成倾向，24小时开始形成新鲜血栓。注入血凝块后5天栓子完全或部分溶解，10天和14天出现机化。与注入血凝块前相比，注入后24小时凝血酶原时间显著缩短[（7.15±0.06）秒]，纤维蛋白原升高[（5.86±1.50）g/L][（7.34±0.19）秒，（3.37±1.02）g/L]（P<0.05）。注入血凝块后5分钟静脉血浆TXA2水平开始升高[（2.5±0.7）μg/L]，15分钟时持续升至最高[（2.5±0.6）μg/L]，然后在60分钟时下降。注入血凝块后5天动脉血和静脉血中的ET水平分别升高[（0.84±0.15）μg/L和（0.23±0.05）μg/L]，而此时大部分栓子已溶解。
自体血凝块诱导的PTE后有血栓形成。此外，血栓形成、纤溶和机化可能始终相互作用，并控制PTE的发病机制。PTE后ET代谢异常，TXA2作为主要介质在PTE早期起重要作用。
