Zhu J, Huang J, Chen Y
Department of Hepatobiliary Surgery, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Zhonghua Wai Ke Za Zhi. 2001 Nov;39(11):875-7.
To study the inhibitory effect of PCNA antisense oligonucleotides or/and VEGF antisense oligonucleotides on growth of human hepatocellular carcinoma in nude mice.
Human hepatocellular carcinoma transplanted subcutaneously in nude mice model was established, and subsequently was divided into four groups: PCNA-ASODN group, VEGF-ASODN group, combined group and control group. Different treatments were given respectively. The weigh and volume of subcutaneous tumors was measured, and the morphological changes of tumor cells was observed under microscope.
The growth inhibitory rate in the PCNA-ASODN group was 72.8%, 44.9% in the VEGF-ASODN group, and 87.2% in the combined group. The combined group which was treated with PCNA-ASODN and VEGF-ASODN showed more effective inhibitory than the PCNA-ASODN group or the VEGF-ASODN group respectively.
The growth of human hepatocellular carcinoma in nude mice can be inhibited by PCNA antisense oligonucleotides and VEGF antisense oligonucleotides respectively. Better inhibitory effect can be obtained if PCNA antisense oligonucleotides and VEGF antisense oligonucleotides are combined in use.
研究增殖细胞核抗原（PCNA）反义寡核苷酸及/或血管内皮生长因子（VEGF）反义寡核苷酸对人肝癌裸鼠移植瘤生长的抑制作用。
建立人肝癌裸鼠皮下移植瘤模型，随后分为四组：PCNA反义寡脱氧核苷酸（ASODN）组、VEGF反义寡脱氧核苷酸组、联合组和对照组，分别给予不同处理。测量皮下肿瘤的重量和体积，并在显微镜下观察肿瘤细胞的形态变化。
PCNA反义寡脱氧核苷酸组的生长抑制率为72.8%，VEGF反义寡脱氧核苷酸组为44.9%，联合组为87.2%。PCNA反义寡脱氧核苷酸与VEGF反义寡脱氧核苷酸联合处理的联合组分别比PCNA反义寡脱氧核苷酸组或VEGF反义寡脱氧核苷酸组显示出更有效的抑制作用。
PCNA反义寡核苷酸和VEGF反义寡核苷酸均可分别抑制人肝癌裸鼠移植瘤的生长。PCNA反义寡核苷酸与VEGF反义寡核苷酸联合使用可获得更好的抑制效果。
