Kordowska-Wiater M, Targoński Z
Department of Food Technology and Storage, Agriculture University, Lublin, Poland.
Acta Microbiol Pol. 2001;50(3-4):291-9.
Karyoductants of Saccharomyces cerevisiae V30 and Pichia stipitis CCY 39501 with the ability to ferment D-xylose to ethanol were isolated. The ability of these isolates to assimilate different sugars, ethanol tolerance and ethanol production from D-xylose was investigated. Karyoductants didn't grow on starch, lactose and cellobiose, like S. cerevisiae, but showed good growth on xylose and L-arabinose, like P. stipitis. All isolates fermented xylose to ethanol slower than P. stipitis and with lower yields, 0.09 - 0.16 g/g. They secreted also about 3.4 - 7.1 g/dm3 of xylitol to the culture medium (P. stipitis only 0.06 g/dm3). The karyoductants showed an average tolerance to ethanol when compared with the parent strains and fermented glucose in the presence of 6% alcohol whereas parent strain S. cerevisiae and P. stipitis showed exogenic ethanol tolerance of 9% and 3%, respectively.
分离出了具有将D-木糖发酵为乙醇能力的酿酒酵母V30和树干毕赤酵母CCY 39501的核融合体。研究了这些分离株同化不同糖类的能力、乙醇耐受性以及从D-木糖生产乙醇的能力。核融合体像酿酒酵母一样，在淀粉、乳糖和纤维二糖上不生长，但像树干毕赤酵母一样，在木糖和L-阿拉伯糖上生长良好。所有分离株将木糖发酵为乙醇的速度都比树干毕赤酵母慢，产量也较低，为0.09 - 0.16克/克。它们还向培养基中分泌了约3.4 - 7.1克/立方分米的木糖醇（树干毕赤酵母仅分泌0.06克/立方分米）。与亲本菌株相比，核融合体对乙醇表现出平均耐受性，在存在6%酒精的情况下能发酵葡萄糖，而亲本菌株酿酒酵母和树干毕赤酵母的外源乙醇耐受性分别为9%和3%。
