Forte T M, Machen T E, Forte J G
Gastroenterology. 1975 Dec;69(6):1208-22.
Neonatal pig gastric mucosa was studied in order to correlate electrophysiological and secretory parameters with ultrastructural changes in membrane components of oxyntic cells. The non-stimulated tissue had a transmucosal resistance of about 130omega - cm2 while the oxyntic cells were characterized by numerous cytoplasmic tubulovesicles and short microvilli extending into patent glandular and canalicular lumina. Upon histamine-stimulation, the average rate of H+ secretion was 8.1 mueq - cm2 - hr-1 and the resistance decreased to 77omega - cm2. The changes were coupled with an immense elaboration of the oxyntic cell apical and canalicular surfaces with a concomitant decrease of tubulovesicles. Thus, the observed decrease in resistance was correlated to large increases in secretory membrane area. Anoxia inhibited H+ secretion while resistance increased to 211omega - cm2. Anoxic oxyntic cells were characterized by swollen mitochondria and occlusion of the lateral intercellular space and basal infoldings. Little change in the configuration of the secretory surfaces was noted, thereby suggesting that restriction of lateral and basal membranes might be responsible for the observed resistance increase. An electrical analogue of gastric mucosa is proposed on the basis of these morphological observations.
为了将电生理和分泌参数与壁细胞的膜成分超微结构变化联系起来，对新生猪胃黏膜进行了研究。未受刺激的组织跨黏膜电阻约为130Ω·cm²，而壁细胞的特征是有许多细胞质微管泡和延伸到开放腺腔和小管腔的短微绒毛。组胺刺激后，H⁺分泌平均速率为8.1μeq·cm²·hr⁻¹，电阻降至77Ω·cm²。这些变化伴随着壁细胞顶端和小管表面的大量细化，同时微管泡减少。因此，观察到的电阻降低与分泌膜面积的大幅增加相关。缺氧抑制H⁺分泌，而电阻增加到211Ω·cm²。缺氧壁细胞的特征是线粒体肿胀以及细胞间侧间隙和基底褶皱闭塞。分泌表面的形态变化不大，这表明侧膜和基底膜的受限可能是观察到的电阻增加的原因。基于这些形态学观察结果，提出了胃黏膜的电模拟模型。
