Truong Khuong, Vielh Philippe, Guilly Marie-Nöelle, Klijanienko Jerzy, Sastre-Garau Xavier, Soussaline Françoise, Dutrillaux Bernard, Malfoy Bernard
Cytogénétique moléculaire et Oncologie, Unité Mixte de Recherche 147, Centre National de Recherche Scientifique-Institut Curie, Paris, France.
Diagn Cytopathol. 2002 Apr;26(4):213-6. doi: 10.1002/dc.10057.
The detection of DNA aneuploid cells using flow cytometry is an indication for the presence of tumor cells, but when DNA diploid cells are found in 25-33% of the cases, the diagnostic and prognostic significance of DNA ploidy is more limited. We analyzed interphase nuclei after in situ hybridization and using image cytometry on 50 breast tumors with diploid DNA content to investigate whether early chromosome rearrangements were detectable and if their occurrence was clinically significant. Imbalances between the two arms of chromosome 1 were found in 55% of the cases and values ranged from 1.5-3.0. Comparison with histological data showed that Grade I tumors mainly have imbalances (67%) and that Grade III tumors were mainly without the imbalance (67%), whereas Grade II tumors were intermediate (50% imbalance). These data suggest that the diagnosis of DNA diploid cases may be improved by using interphase FISH. In addition, the data also indicates that early breast tumors may have different genetic origins, which is important in the comprehension of tumor malignancy in early stages, especially for preinvasive lesions.
使用流式细胞术检测DNA非整倍体细胞提示存在肿瘤细胞，但当在25% - 33%的病例中发现DNA二倍体细胞时，DNA倍性的诊断和预后意义更为有限。我们对50例DNA含量为二倍体的乳腺肿瘤进行原位杂交后分析间期核，并使用图像细胞术研究是否可检测到早期染色体重排及其发生是否具有临床意义。55%的病例中发现1号染色体双臂之间存在不平衡，数值范围为1.5 - 3.0。与组织学数据比较显示，I级肿瘤主要存在不平衡（67%），III级肿瘤主要无不平衡（67%），而II级肿瘤为中间情况（50%不平衡）。这些数据表明，通过使用间期荧光原位杂交（FISH）可改善DNA二倍体病例的诊断。此外，数据还表明早期乳腺肿瘤可能具有不同的遗传起源，这对于理解早期肿瘤的恶性程度很重要，尤其是对于原位癌前病变。
