Benes Martin, Billy Didier, Hermens Wim T, Hof Martin
J. Heyrovský Institute of Physical Chemistry, Academy of Science of the Czech Republic, Centre for Complex Molecular Systems and Biomolecules, Prague.
Biol Chem. 2002 Feb;383(2):337-41. doi: 10.1515/BC.2002.037.
We demonstrate for the first time that ellipsometry and confocal fluorescence correlation spectroscopy (FCS) are complementary methods for the characterisation of supported planar phospholipid bilayers (SPBs) formed on mica, a mineral used in atomic force microscopy investigations of SPBs. Addition of small unilamellar vesicles containing 20% dioleoyl-phosphatidylserine (DOPS) and 80% dioleoyl-phosphatidylcholine (DOPC) to an oxidised borosilicate surface, on the other hand, results in a planar lipid system characterised by lateral diffusion coefficients which are three time smaller than those obtained for SPBs. Moreover, seven labelled phospholipids were tested for their suitability in the FCS characterisation of vesicles as well as of SPBs.
我们首次证明，椭圆偏振光谱法和共聚焦荧光相关光谱法（FCS）是用于表征在云母上形成的支撑平面磷脂双层（SPB）的互补方法，云母是一种用于SPB原子力显微镜研究的矿物。另一方面，向氧化硼硅酸盐表面添加含有20%二油酰磷脂酰丝氨酸（DOPS）和80%二油酰磷脂酰胆碱（DOPC）的小单层囊泡，会形成一种平面脂质体系，其横向扩散系数比SPB的横向扩散系数小三倍。此外，还测试了七种标记磷脂在囊泡以及SPB的FCS表征中的适用性。
