Uguen Marilyne, Uguen Patricia
Laboratoire de Biologie et Chimie Moléculaires, EA 2594, Université de Bretagne Sud, Centre de Recherche Yves Coppens, P.O. Box 573, 56017, Vannes, France.
FEMS Microbiol Lett. 2002 Feb 19;208(1):99-103. doi: 10.1111/j.1574-6968.2002.tb11067.x.
Lacticin 481 is produced by Lactococcus lactis subsp. lactis and belongs to subgroup AII of the lanthionine-containing bacteriocins. The putative homodimeric LctT involved in lacticin 481 production shares significant similarities with the 'LcnC' protein encoded by 'lcnC', located on the chromosome of the lactic acid bacterium, L. lactis IL1403. LctT and 'LcnC' belong to the recently defined family of AMS (ABC transporter maturation and secretion) proteins. Inactivation of the 'lcnC' gene demonstrates that it is not responsible for the weak lacticin 481 production observed in a strain expressing only the precursor peptide LctA, and the modification enzyme LctM. This result indicates that the two AMS proteins, 'LcnC' and LctT, are not interchangeable in the machinery of processing/export of lacticin 481.
乳酸链球菌素481由乳酸乳球菌乳酸亚种产生，属于含羊毛硫氨酸的细菌素AII亚组。参与乳酸链球菌素481产生的假定同型二聚体LctT与位于乳酸乳球菌IL1403染色体上的由“lcnC”编码的“LcnC”蛋白具有显著相似性。LctT和“LcnC”属于最近定义的AMS（ABC转运蛋白成熟与分泌）蛋白家族。“lcnC”基因的失活表明，它与仅表达前体肽LctA和修饰酶LctM的菌株中观察到的乳酸链球菌素481产量低无关。这一结果表明，两种AMS蛋白“LcnC”和LctT在乳酸链球菌素481的加工/输出机制中不可互换。
